Animal Reproduction Science 134 (2012) 164–176 Contents lists available at SciVerse ScienceDirect Animal Reproduction Science journa l h omepa g e: www.elsevier.com/locate/anireprosci Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs Jung-Il Chae a,1 , Jumi Kim b,1 , Seong Goo Lee c , Min Whan Koh c , Young-Joo Jeon a , Dong-Wook Kim a , Seon mi Ko a , Kang Seok Seo d , Hak Kyo Lee e , Nag-Jin Choi f , Seong-Keun Cho g , Joohyun Ryu h , Sunghyun Kang h , Dong-Seok Lee i , Hyung-Min Chung b,∗∗ , Deog-Bon Koo j,∗ a Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 Project, Chonbuk National University, Jeonju 651-756, Republic of Korea b CHA Bio & Diostech Co., Ltd., 606-16 Yeoksam 1 dong, Gangnam gu, Seoul 135-907, Republic of Korea c Department of Obstetrics and Gynecology, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea d Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Republic of Korea e Genomic Informatics Center, HankyongNationalUniversity, 67 Sukjong-dong, Ansung-city, Kyongi-do 456-749, Republic of Korea f Department of Animal Science, College of Agricultural & Life Science, Chonbuk National University, Jeonju, Republic of Korea g Department of Animal Science, College of National Resources and Life Science, Pusan National University, Miryang-si, Gyeongnam 627-706, Republic of Korea h Medical Proteomics Research Center, KRIBB, Daejeon, Republic of Korea i College of Natural Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea j Department of Biotechnology, College of Engineering, Daegu University, 15 Jillyang Gyeongsan, Gyeongbuk 712-714, Republic of Korea a r t i c l e i n f o Article history: Received 16 September 2011 Received in revised form 17 May 2012 Accepted 13 July 2012 Available online 31 July 2012 Keywords: Development Peripheral blood mononuclear cells Pig Pregnancy Proteomic a b s t r a c t Information obtained from peripheral blood could help us understand the underlying mech- anisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC–MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large num- ber of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S- transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC–MS/MS was applied to evaluate specific molecular expression changes during preg- nancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy. © 2012 Elsevier B.V. All rights reserved. ∗ Corresponding author at: Department of Biotechnology, College of Engineering, Daegu University, 15 Jillyang Gyeongsan, Gyeongbuk 712-714, Republic of Korea. Tel.: +82 53 850 6557; fax: +82 53 850 6559. ∗∗ Corresponding author at: Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University, 605-21 Yeoksam 1 dong, Gangnam gu, Seoul 135-907, Republic of Korea. Tel.: +82 2 3468 3391; fax: +82 2 3468 3373. E-mail addresses: hmchung@cha.ac.kr (H.-M. Chung), dbkoo@daegu.ac.kr (D.-B. Koo). 1 Jung-Il Chae and Jumi Kim contributed equally to this work. 0378-4320/$ – see front matter © 2012 Elsevier B.V. All rights reserved. http://dx.doi.org/10.1016/j.anireprosci.2012.07.008