TETRAHEDRON: ASYMMETRY Tetrahedron: Asymmetry 14 (2003) 689–700 Pergamon Asymmetric synthesis of 2H -aziridine phosphonates, and - or -aminophosphonates from enantiomerically enriched 2H -azirines Francisco Palacios,* Domitila Aparicio, Ana Marı ´a Ochoa de Retana, Jesu ´s M. de los Santos, Jose ´ I. Gil and Rafael Lo ´ pez de Munain Departamento de Quı ´mica Orga ´nica I, Facultad de Farmacia, Universidad del Paı ´s Vasco, Apartado 450, 01080 Vitoria, Spain Received 31 October 2002; revised 9 January 2003; accepted 22 January 2003 Abstract—A simple and efficient method for asymmetric synthesis of 2H-azirine-2-phosphonates 6 is described. The key step is a base-mediated Neber reaction of p -toluenesulfonyloximes 4 derived from phosphonates. Triethylamine 5, alkaloids 7 and solid-phase bound achiral 8 or chiral amines 9 are used. Reduction of 2H-azirines 6 with sodium borohydride in ethanol gives cis -aziridine-phosphonates 10. Ring opening of aziridines 10 and 11 leads to the formation of enantiomerically enriched -12 and 14 and -aminophosphonates 13 and 15. © 2003 Elsevier Science Ltd. All rights reserved. 1. Introduction 2H -Azirine ring systems represent an important class of compounds because of their high reactivity. 1 They can be used as key intermediates in organic synthesis in the preparation of acyclic functionalized amino derivatives 2a–c and heterocycles 2d–h since all three bonds of the azirine ring can be cleaved, depending on the experimental conditions used. In particular, 2H -azirines containing a carboxylic ester group (I, Fig. 1) are constituents of naturally occurring antibiotics 1a and are excellent reagents for the preparation of functionalized aziridines 1,3 and - 3b,4a–e and -amino acid deriva- tives. 3b,4f–h Furthermore, fosfomycin IIIa (X=O) (Fig. 1), a three ring heterocycle containing a phosphorus substituent is a broad spectrum antibiotic, 5a,b which has been used in vitro 5c,d and clinically. 5e It is also known that phosphorus substituents regulate important biolog- ical functions, 6 and that molecular modifications involving the introduction of organophosphorus func- tionalities could increase their biological activity in a similar manner to that reported for other pharmaceuti- cals. 6 Some procedures for the synthesis of 2H -azirines have been reported, 1 and enantiomerically enriched 2H - azirine carboxylates I have been prepared from chiral N -substituted aziridine 2-carboxylic esters, 7a,b and by using the Neber reaction. 7c,d However, despite the potential utility of 2H -azirines, 1 these three-membered heterocycles directly substituted with a phosphorus con- taining functional group have received little attention. 8 For these reasons, the development of new processes for the asymmetric synthesis of functionalized azirines containing a phosphorus substituent at the 2 position (II, Fig. 1) may represent an important tool in organic synthesis because these heterocycles are expected to play a similar role to that of their isosteric analogues I, and therefore could be used as starting materials for the preparation of phosphorus substituted aziridines IIIb and in the enantioselective synthesis of - and - aminophosphorus derivatives. -Aminophosphonates 9 can be considered as surrogates for -amino acids, 10a and have been used as haptens for the generation of catalytic antibodies, 10b,c as antibacterial agents, 10d,e and as nucleosides, 10f or as phosphapeptide enzyme inhibitors, 10g–k whereas -aminophosphonate deriva- tives, some of them naturally occurring, 9b,11a–c have been used for the preparation of hydroxy deriva- tives, 12a–c of peptide-based enzyme inhibitors 12d–g and as agrochemicals 12h or pharmaceuticals. 12i–k Figure 1. * Corresponding author. Tel.: +34-945-013103; fax: +34-945-013049; e-mail: qoppagaf@vf.ehu.es 0957-4166/03/$ - see front matter © 2003 Elsevier Science Ltd. All rights reserved. doi:10.1016/S0957-4166(03)00089-2