DOI: 10.1002/adma.200600200 Dissolving Behavior and Stability of ZnO Wires in Biofluids: A Study on Biodegradability and Biocompatibility of ZnO Nanostructures** By Jun Zhou, Ningsheng Xu, and Zhong L. Wang* Fabrication of nanoscale biosensors based on nanowires (NWs), [1–5] nanotubes (NTs), [6–9] and other nanomaterials [10] has recently attracted enormous attention. In comparison to nanoparticles, 1D NWs and NTs have higher sensitivity be- cause of depletion or accumulation of charge carriers at the surface that is caused by binding of charged biological macro- molecules at the surface, and affects the entire cross-sectional conduction pathway. [4] Among all 1D nanomaterials, Si NWs and carbon NTs are the most studied materials as biosensors. Functionalized Si NWs and carbon NTs have been demon- strated for detecting proteins, [2] DNA and DNA sequence variations, [4] and cancer markers. [3] However, the biocompat- ibility and biodegradability of these nanostructures remain to be studied. For example, carbon NTs injected into human blood vessels might accumulate and occlude capillaries in the human brain, which could cause serious damage or be fatal. Being a key functional material with versatile properties, such as dual semiconducting and piezoelectric properties, ZnO has important applications in optoelectronic devices, sensors, lasers, transducers, and photovoltaic devices. [11–13] In addition, the morphology [12,14–18] and the dopant concentra- tion [19] of ZnO nanostructures can be well controlled by tun- ing the growth conditions, which further broadens their appli- cations. ZnO nanoparticles are believed to be nontoxic, bio- safe, and possibly biocompatible, and have been used in many applications in our daily life, such as drug carriers and cos- metics. However, no literature is available on the biodegrad- ability and biocompatibility of ZnO nanowires or nanobelts, which is crucial for the application of ZnO nanostructure for biosensing. In this paper, we present the first study on biodegradability and biocompatibility of ZnO wires. We have conducted a sys- tematic study on the etching and dissolving behavior of ZnO NWs in various solutions with moderate pH values, including deionized water, ammonia, NaOH solution, and horse blood serum. The result shows that ZnO can be dissolved by deion- ized water (pH ≈ 4.5–5.0), ammonia (pH ≈ 7.0–7.1, 8.7–9.0) and NaOH solution (pH ≈ 7.0–7.1, 8.7–9.0). The study of the interaction of ZnO wires with horse blood serum shows that the ZnO wires can survive in the fluid for a few hours before they eventually degrade into mineral ions. The results of this study are of great significance. First, biosensors made of ZnO nonmaterial have a certain time to perform a device function. Secondly, once completing the corresponding service, the ZnO wires can eventually dissolve into ions that can be com- pletely absorbed by the body and become part of the nutri- tion. The biodegradability and biocompatibility of ZnO NWs would allow their use for in vivo biosensing and biodetection. Synthesized by a vapor–solid growth process, [12] the ZnO wires used in our study grew along the [0001] direction with a hexagonal cross section and were of high crystalline quality. We studied the dissolving behavior of ZnO wires in deionized water (pH ≈ 4.5–5.0), ammonia (pH ≈ 7.0–7.1, 8.7–9.0), NaOH solution (pH ≈ 7.0–7.1, 8.7–9.0), horse blood serum solution (pH ≈ 7.9–8.2), and pure horse blood serum (pH ≈ 8.5). The two kinds of ammonia used in our study were prepared by di- luting concentrated ammonia with deionized water. The two kinds of NaOH solution were prepared by dissolving solid NaOH in deionized water, and the horse blood serum solution was prepared by diluting pure horse blood serum with NaOH solution (pH ≈ 7.0–7.1) with a volume ratio of 1:10. We adopted two processes to investigate the dissolving be- havior of a single ZnO wire in different liquids. To study the dissolving process of ZnO wires in deionized water, ammonia, and NaOH solution, we used Process 1 illustrated in Fig- ure 1a. Individual ZnO NWs were firstly manipulated with a pin and placed on a silicon substrate. After that, a droplet of COMMUNICATIONS 2432 © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Adv. Mater. 2006, 18, 2432–2435 – [*] Prof. Z. L. Wang, J. Zhou School of Materials Science and Engineering Georgia Institute of Technology Atlanta, GA 30332-0245 (USA) E-mail: zhong.wang@mse.gatech.edu J. Zhou, Prof. N. S. Xu School of Physics and Engineering State Key Lab of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Materials and Technologies Sun Yat-Sen (Zhongshan) University Guangzhou 510275 (P.R. China) [**] Z. L. Wang thanks the support from NSF, the NASA Vehicle Sys- tems Program and Department of Defense Research and Engineer- ing (DDR&E), and the Defense Advanced Research Projects Agency (Award No. N66001-04-1-8903). N. S. X. thanks the support of the project from the NSF of China (Grant No. 50 021 202, 50 329 201, and 90201 020), Ministry of Science and Technology of China (Grant No. 2003CB314700 and 2002AA313010), Ministry of Educa- tion of China, Science and Technology Department of Guangdong Province, Education Department of Guangdong Province, and Science and Technology Department of Guangzhou City. J. Zhou thanks the KAISI FUND from SunYat-Sen (Zhongshan) University. We thank the help of Y. Ding, Rusen Yang, Jin Liu, Wenjie Mai, Y. Y. Zhang, and Gang Bao. Supporting Information is available online from Wiley InterScience or from the author.