The changing of enzymatic acti matrix for purulent and bu Dosadina Elina Eldarovna 1,* , Kulmety 1 D.I.Mendeleev University of Chemical Technology of Russ ABSTRACT This research is devoted to creating and studyin enzymes for wound healing. We studied differen technological features of materials production), conditions). Also we proposed a structural sch altogether. The understanding of this scheme w properties in future. Keywords: chitosan, chitosan-coated dialdehyd 1. INTRODUCTION The problem of creating of the medical m healing is still relevant these days. There is a lot devoted to the designing of cellulose deriv enzymes and other therapeutic agents, the scientific base of the production of such technological features of this process, the study and new fields of these materials application [1-3 Modern wound dressing materials are qu the traditional ones by their properties and d wound dressings appear to be not only textile various powders, films, sponges, gels and com things [2,3]. The application of biodegradable p the most relevant trends in the designing of products [1,3-6]. It should be noticed that wo disposable materials with a small exploitation hours) so the biological activity should be comp this time [1]. The objects of many researches today based on natural polysaccharide – cellulose an Great potential of the cellulose fibers is related structure that offers magical possibilities as creating of the bioactive, biocompatible and so- materials [1,5,7]. The features of exploitatio dressings should determine the selection of poly of immobilization. The drugs based on the enzymes for wou been used for a long time [1]. Proteolytic enzym 2. EXPERIMENTAL SECTION 2.1. Materials and methods. 2.1.1. Reagents. In this work we used: collitin (Col) – p of enzymes from pancreas of pig (VFS42 proteolytic complex from hepatopancreas of cra Schelkovo, Moscow region, Russia; trypsin (Tr Volume 6, Issue 3, 2016, 1291-1298 Original Research Article Biointerface Res www ivity of hydrolases immobilized on n urn wounds treatment during storing yeva Margarita Anatolyevna 1 , Belov Alexe sia (MUCTR), dep. Biotechnologies; Research Institute of Textile M *corresponding author ng of special properties of modified cellulose medica nt parameters of materials production (glycerin additi the changing of their properties during storing and heme of obtained materials and mechanisms of inte will lead to scientifically based designing of the new w e cellulose, immobilized enzymes, proteinases, wound materials for wound of new researches vatives containing development of materials, special y of the properties 3]. uite different from design. Nowadays materials but also mbinations of those polymers is one of new wound care ound dressings are n term (about 72 pletely realized by are the materials nd its derivatives. d to the molecular a carrier for the -called intellectual on of the wound ymer and the way und treatment have mes could clean the wound from the exudate, provid wound and also decrease microorganisms on the woun secondary infection [8,9]. In no is a trend of using mostly poly- pure medical enzymes. Poly-en of different enzymes what hel better by affecting different che destroying them. Also the cost far lower than the cost of pure e enzyme high purification stag healing drugs production [1, 9-1 One more substance that medicine for wound care for v are a lot of studies where chitos therapeutic agents [9,11] or antibacterial properties ag Staphylococcus aureus - microo percentage of the pathogenic mi 13]. Chitosan is a biodegradable In this article we d immobilized materials for woun and general characteristics an scheme of obtained materials. roteolytic complex 2-2483-95); PC – ab (“Bioprogress”, r) – from pancreas of cattle (“Spofa”, Czech Rep Schelkovo, Moscow region, 00472124-03, deacetylation 383.7 cSt, MW 478 kDa). All analytical grade. Received: 27.04.2016 / Revised: 15.05.2016 / Accepte search in Applied Chem w.BiointerfaceResearch.com Page | 1291 natural polysaccharide g and exploitation ey Alexeevich 1 Materials, Moscow, Russian Federation e-mail address: elinadosadina@gmail.com al materials based on immobilized ion during immobilization process, exploitation (effect of temperature eraction of all of the components wound care materials with requires healing, drug design. de a normal blood circulation in the e the number of pathogenic nd surface, which could cause a owadays in modern medicine there -enzymatic preparations rather than nzymatic drugs contain a complex lps to hydrolyze a wound exudate emical bonds in wound exudate and of poly-enzymatic preparations is enzymes because of the lack of the ge in the process of the wound 10]. t is also used quite often in modern various purposes is chitosan. There san is used as a carrier for different as an active compound with gainst Escherichia coli and organisms that make up the largest icroflora on the wound surface [11- e and biocompatible polymer. designed different compositions of nd healing, studied their properties nd proposed a possible structural public); chitosan (Ct) (“Bioprogress”, Russia, humidity 10%, 9289-067- degree 80.0%, kinematic viscosity l chemicals were used as received as ISSN 2069-5837 Open Access Journal ed: 25.05.2016 / Published on-line: 15.06.2016 mistry