Int.J.Curr.Microbiol.App.Sci (2017) 6(4): 2037-2047 2037 Original Research Article https://doi.org/10.20546/ijcmas.2017.604.241 Biofilm Formation of Zinc Solubilizing, Potassium Releasing Bacteria on the Surface of Fungi Y. Nagaraju*, S. Triveni, R. Subhashreddy and P. Jhansi Department of Agricultural Microbiology, College of Agriculture, Professor Jayashankar Telangana State Agricultural University (PJTSAU), Rajendranagar, Hyderabad-30, India *Corresponding author ABSTRACT Introduction Biofilms are the common life strategy for bacteria in natural environments. Biofilms are composed of populations or communities of microorganisms embedded in self-produced polymeric matrix (mainly extracellular polysaccharides) that have adhered to environmental surfaces in which sufficient moisture is present (Costerton et al., 1995). These three-dimensional microbial communities may be formed in all environments colonized by bacteria, such as on solid substrates in contact with moisture or on tissue surfaces in living organisms. The mutualistic association between microbial communities and plant roots, the so-called rhizosphere, form an environment that fulfils the requisites for biofilm formation: sufficient moisture and a supply of nutrients, which are provided by the plant. Most researchers working with rhizospheric bacteria have not described the formation of biofilms on plant roots. In the past, however, different reports International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 4 (2017) pp. 2037-2047 Journal homepage: http://www.ijcmas.com Attachment of soil bacteria to plant cells is supposedly the very early step required in plant–microbe interactions. Attachment also is an initial step for the formation of microbial biofilms on plant roots. For the rhizobia–legume symbiosis, various mechanisms and diverse surface molecules of both partners have been proposed to mediate in this process. A biofilm is an aggregate of microorganisms in which cells are stuck to each other and/or to biotic/abiotic surface. These adherent cells are frequently embedded within a self- produced matrix of Extracellular Polymeric Substance (EPS) is a polymeric jumble of DNA, proteins and polysaccharides. The high population density achieved in biofilms provides the opportunity to perform enhanced biochemical reactions than that of single isolates and dual cultures. In the present investigation we aimed towards the development of biofilms under in vitro conditions, using a combination of agriculturally important potential microorganisms like zinc solubilizing, potassium releasing bacteria on the fungal hyphosphere. All the isolates were screed for their zinc solubilization as well as potassium releasing capacity along with biochemical characterization. Biofilm formation of zinc solubilizing and potassium releasing isolates has considerably enhanced the release of potassium as well as zinc. Four hyphobacteria has exhibited high potential for solubilization of organic Zn to inorganic Zn as well as potassium (K) released considerably high when biofilm is used. Keywords Mango pulp, Mango peel powder (MPP), Nectar, Chemical preservative, Sensory properties. Accepted: 15 March 2017 Available Online: 10 April 2017 Article Info