International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395-0056 Volume: 04 Special Issue: 09 | Sep -2017 www.irjet.net p-ISSN: 2395-0072 One Day International Seminar on Materials Science & Technology (ISMST 2017) 4 th August 2017 Organized by Department of Physics, Mother Teresa Women’s University, Kodaikanal, Tamilnadu, India © 2017, IRJET | Impact Factor value: 6.171 | ISO 9001:2008 Certified Journal | Page 390 Anti-cancer and Cytotoxic studies on DAST crystal J. Arul Martin Mani 1 , V. Joseph 1 , P. Sagayaraj 1* 1 Department of Physics, Loyola College, Chennai 600034, India ---------------------------------------------------------------------***---------------------------------------------------------------------- Abstract - The title compound 4-N,N-dimethylamino-Ͷ’- N’-methyl-stilbazolium tosylate (DAST) has been synthesized by metathesization of 4-N,N-dimethylamino- Ͷ’-N’-methyl stilbazolium iodide (DMSI) and sodium p- toluene sulfonate. Single crystal X-ray diffraction analysis confirms the unit cell parameters of the grown crystal. The present study was designed to investigate DAST for its anticancer activity on human breast cancer cells and cytotoxic effect. The results show strong inhibition against the MCF-7 cell lines and weak inhibition against the Vero cell lines which makes it suitable for pharmaceutical applications. Key Words: DAST, Anticancer and cytotoxic activity 1. INTRODUCTION Cancer is the major cause of mortality in the world. Reports say that breast cancer is one of the most common types of cancers in females. Though there are commonly used methods for the treatment of cancer, still there is a significant need to improve current cancer therapies and search for novel compounds [1-2]. Cell viability and cytotoxicity analysis are used for drug screening and to test new compounds [3]. The works on anticancer and cytotoxic activity measurements of various samples by MTT assay method has been reported [4-5]. Heterocyclic derivatives, especially pyridine based crystals were reported as potential materials for biological activities [6]. In this study, anticancer and cytotoxic activities of DAST against MCF-7 and Vero cell lines are investigated by MTT assay method. 2. EXPERIMENTAL PROCEDURE 2.1 Synthesis DAST was prepared by metathesization of 4-N,N- dimethylamino-Ͷ’-N’-methyl stilbazolium iodide (DMSI) salt with sodium p-toluene sulfonate. DMSI was prepared by the condensation of 1,4-dimethyl pyridinium iodide (2.35 g, 10 mmol), methanol (30 ml) and 4-N, N- dimethylamino benzaldehyde (1.79 g, 10 mmol) in the presence of piperidine (0.2 ml) [7]. DMSI (0.7324 g, 2 mmol) and sodium p-toluene sulfonate (0.3883 g, 2 mmol) were simultaneously dissolved in 70 ml and 30 ml of Millipore water respectively at 70 °C. These two hot solutions were mixed and heated for 30 minutes at 70 °C and then allowed to cool naturally to room temperature. A red precipitate was obtained as a result of exchange reaction between anion and cation. Supersaturated solution of this dried salt was prepared in methanol and crystals were grown by slow evaporation technique. The photograph of the grown crystal is shown in Fig. 1. Fig - 1: Photograph of grown DAST single crystal 3. RESULTS AND DISCUSSION 3.1 Single crystal XRD XRD analysis for the grown crystal has been carried out to identify the cell parameters using an ENRAF NONIUS CAD 4 automatic X-ray diffractometer with MoKȽ ȋλ=Ͳ.͹ͳ͹Ͳ ÅȌ. The lattice parameters are a = 10.3648 Å, b = 11.3224 Å, c = 17.8933 Å, Ƚ = γ = 90°, Ⱦ = 92.24° and the grown crystal belongs to monoclinic system with space group Cc, which very well matches with the reported value [8]. 3.2 Anticancer activity of DAST The MCF-7 Cells (human breast cancer cells) were plated in 24-well plates (1 × 10 5 /well) and incubated in 37°C with 5% CO2 condition. After the cell reaches the confluence, the various concentrations of the samples were added and incubated for 24 hrs. After incubation, the sample was removed from the well and washed with phosphate-buffered saline (pH 7.4) or MEM without serum. 100 µl/well (5 mg/ml) of 0.5 % 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) was added and incubated for 4 hours. After incubation, 1 ml of DMSO was added in all the wells. The absorbance at 570