Citation: Rieznichenko LS, Gruzina TG, Dybkova SM, Ushkalov VO and Ulberg ZR. Investigation of Bismuth Nanoparticles Antimicrobial Activity against High Pathogen Microorganisms. American J Bioterror Biosecur Biodefens. 2015;2(1): 1004. American J Bioterror Biosecur Biodefens-Volume 2 Issue1 - 2015 Submit your Manuscript | www.austinpublishinggroup.com Rieznichenko et al. © All rights are reserved American Journal of Bioterrorism, Biosecurity and Biodefense Open Access Abstract Spherical bismuth nanoparticles with average size 40 nm have been synthesized by the colloidal-chemical method in water medium. 100% of Bi content has been revealed in the nanoparticles by the method of energy- dispersive X-ray spectroscopy. Synthesized bismuth nanoparticles have been characterized as biosafe and biocompatible according to the Guidelines «Safety assessment of medical nanopreparations». Bismuth nanoparticles’ antimicrobial activity in vitro has been estimated against wide spectra of high pathogen microorganisms - potential causative agents of human and animals’ diseases. Bismuth nanoparticles’ high bactericidal action against all investigated test strains: Campylobacter jejuni Pl – 09.c; Listeria monocytogenes АТСС 19112; Yersinia enterocoliticа 12/15-08; Salmonella typhimurium №16; Escherichia coli №4; Mycoplasma arginini G 230; Acholeplasma laidlawii ATCC 23206; Bacillus anthracis M-71; Leptospira Pomona has been revealed. Keywords: Bismuth nanoparticles; Synthesis; Antimicrobial activity; Pathogens and size of synthesized BiNP have been deined by the method of Transmission Electron Microscopy (TEM) (JEM-1230 «JEOL LTD», Japan). he method of energy-dispersive X-ray spectroscopy has been used for chemical composition’s X-ray microanalysis of the synthesized nanoparticles (IETEM 250 with detector X-Max 80, Oxford Instruments Analytical, UK for JEM-1230). BiNP biosafety level has been estimated in vitro using parameters of cytotoxicity, genotoxicity, mutagenicity and biochemical markers according to the Guidelines «Safety assessment of medical nanopreparations» [4]. High pathogen strains - causative agents of most dangerous human and animals’ diseases: Campylobacter jejuni Pl – 09.c; Listeria monocytogenes АТСС 19112; Yersinia enterocoliticа 12/15- 08; Salmonella typhimurium №16; Escherichia coli №4; Mycoplasma arginini G 230; Acholeplasma laidlawii ATCC 23206; Bacillus anthracis M-71; Leptospira Pomona from the Collection of he State Scientiic Abbreviations BiNP: Bismuth Nanoparticles Introduction Despite of the common eforts in biodefense around the world the problem of high dangerous pathogens distribution, especially under the threat of terrorist attacks, is still urgent. Among the biotoxins that could be used for dispersion, leading experts identify large number of high pathogen microorganisms of category A and B, such as well- known pathogens: Bacillus anthracis, Salmonella, Escherichia etc.[1- 3]. he most important problem in the struggle against biohazard is high efective defense of civil population. Such efective defense can be provided using safe drugs, which are efective against wide range of biotoxins. Modern antimicrobial preparations and vaccines don’t possess by wide range of activity against diferent classes of pathogens. So, the search and development of new high efective antimicrobial substances with high efectiveness against wide spectra of high pathogen microorganisms - causative agents of most dangerous human and animals’ diseases is topical problem today. Metal nanoparticles are possessed by high potential in this area. he main goal of presented work was antimicrobial activity investigation of the synthesized bismuth nanoparticles against wide spectra of pathogen microorganisms - potential causative agents of high dangerous human and animals’ diseases. Materials and Methods Spherical Bismuth Nanoparticles (BiNP) have been synthesized by the method of chemical condensation in water medium. he concentration of obtained BiNP was 77.5 mg/ml by metal. he shape Research Article Investigation of Bismuth Nanoparticles Antimicrobial Activity against High Pathogen Microorganisms Rieznichenko LS 1 *, Gruzina TG 1 , Dybkova SM 1 , Ushkalov VO 2 and Ulberg ZR 1 1 Department of Colloidal Technology of Natural Systems, F.D. Ovcharenko Institute of Biocolloidal Chemistry, Ukraine 2 State Scientiic Control Institute of Biotechnology and Strains of Microorganisms, Ukraine *Corresponding author: Rieznichenko LS, Department of Colloidal Technology of Natural Systems, F.D. Ovcharenko Institute of Biocolloidal Chemistry, Vernadskogo Av., 42, 03142 Kyiv, Ukraine Received: October 01, 2014; Accepted: February 02, 2015; Published: February 04, 2015 Figure 1: Transmission electron microscope image of synthesized spherical bismuth nanoparticles with average particles’ size 40 nm.