ISSN: 2320-5407 Int. J. Adv. Res. 5(11), 138-142 138 Journal Homepage: -www.journalijar.com Article DOI:10.21474/IJAR01/5747 DOI URL: http://dx.doi.org/10.21474/IJAR01/5747 RESEARCH ARTICLE DNA EXTRACTION AND PCR DIAGNOSIS OF BREAST CANCER PATIENTS AMONG THE POPULATION OF TRIPURA. * Asis Debbarma 1 , Pradip Kumar Ghosh 2 , Subhojit Das 1 , Anubha Nandi Kar 3 and Biswajit Chowdhury 3 . 1. Assistant Professor, Department of Biochemistry, Agartala Govt. Medical College, Agartala, Tripura West, India. 2. Associate Professor, Department of Biochemistry, Agartala Govt. Medical College, Agartala, Tripura West, India. 3. Junior Research Fellow, DBT Project, Department of Biochemistry, Agartala Govt. Medical College, Agartala, Tripura West, India. …………………………………………………………………………………………………….... Manuscript Info Abstract: ……………………. ……………………………………………………………… Manuscript History Received: 02 September 2017 Final Accepted: 04 October 2017 Published: November 2017 Key words:- BRCA1, BRCA2, Breast Cancer,PCR. Breast cancer is a malignant tumor that starts in the cells of breast. A malignant tumor is a group of cancer cells that can grow into (invade) surrounding tissues or spread to distant areas (metastasize) of the body. Several environmental risk factors that may contribute to or hasten the development of breast cancer have been identified including mainly lifestyle, reproductive factors and family history. Mutations in the BRCA1 and BRCA2 genes profoundly increase the risk of developing breast cancer among women. BRCA1 gene is located on chromosome No. 17. The normal BRCA1 genes encode 1863 amino acids. BRCA2 gene located on chromosome No. 13 is another DNA repair gene. The normal BRCA2 genes encode 3418 amino acids. To explore the contribution of BRCA1 and BRCA2 mutations in the development of breast cancer among Indian women, we carried out DNA extraction & PCR analysis of the BRCA1 and BRCA2 genes in thirty eight (38) number of breast cancer female patients from Tripura, India. Copy Right, IJAR, 2017,. All rights reserved. …………………………………………………………………………………………………….... Introduction:- Genetic predisposition for familial early-onset breast cancer accounts for approximately 5–10% of all breast cancers and 7–10% of all ovarian cancers [1]. Mutations in two autosomal dominant genes BRCA1 and BRCA2 have been linked to familial breast or breast and ovarian cancer [2-4]. Women who carry BRCA1 or BRCA2 genes mutations have an estimated lifetime risk of between 60% and 85% for developing breast cancer and a lifetime risk of between 26% and 54% for developing ovarian cancer for BRCA1, and between 10% and 23% for BRCA2 [5-8]. Every year 75,000 new cases of breast cancer are diagnosed in Indian women [9]. In India, breast cancer accounts for about 19- 34% of all cancer cases among women. As per national and regional cancer registries, it is the commonest cancer amongst women in Delhi, Mumbai, Ahmedabad, Kolkata and Trivandrum [10-12]. A recent study on breast cancer risk in India, revealed that one in twenty eight women develop breast cancer during her life time. This is higher in urban areas. In Tripura state too breast cancer is the second leading site among females, the first and third being carcinoma cervix and lungs [13]. Because of the continuing rise in breast cancer incidence, an urgent need to develop strategies for prevention is very much required. Carcinoma of breast has a complex etiology some of which are hormonal, genetic and environmental factors operating over a long period [14]. Corresponding Author: - Dr. Asis Debbarma. Address: - Assistant Professor, Department of Biochemistry, Agartala Govt. Medical College, PO: Kunjaban-799006, Agartala, Tripura West, India.