e297 Med Oral Patol Oral Cir Bucal. 2010 Mar 1;15 (2):e297-302. Epstein Barr Virus detection in oral hairy leukoplakia Journal section: Oral Medicine and Pathology doi:10.4317/medoral.15.e297 Publication Types: Research Epstein Barr Virus detection and latent membrane protein 1 in oral hairy leukoplakia in HIV + Venezuelan patients Xiomara González 1 , María Correnti 2 , Helen Rivera 3 , Marianella Perrone 4 1 Graduate Student Stomatology Program 2 Associate Professor, Head of Molecular Genetic Laboratory.Instituto de Oncología y Hematología, Ministerio del Poder Popular Para la Salud, Instituto de Investigaciones Odontológicas Dr. Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela 3 Full Professor, Director Instituto de Investigaciones Odontológicas, Head of Oral Pathology Laboratory, Facultad de Odonto- logía, Universidad Central de Venezuela 4 Full Professor. Instituto de Investigaciones Odontológicas Dr. Raúl Vincentelli, Universidad Central de Venezuela Correspondence: Laboratorio de Genética Molecular Instituto de Oncología y Hematología MPPS- 58-212-6050647 Caracas, Venezuela mcorrentip@yahoo.com Received: 11/12/2008 Accepted: 28/11/2009 González X, Correnti M, Rivera H, Perrone M. Epstein Barr Virus detec- tion and latent membrane protein 1 in oral hairy leukoplakia in HIV+ Venezuelan patients. Med Oral Patol Oral Cir Bucal. 2010 Mar 1;15 (2):e297-302. http://www.medicinaoral.com/medoralfree01/v15i2/medoralv15i2p297.pdf Abstract Purpose: To determine the prevalence of Epstein Barr Virus (EBV) in oral hairy leukoplakia lesions (OHL) in HIV+ Venezuelan patients. Material and Method: In this case study, we evaluated 21 HIV+ adult patients with clinically present OHL lesions, 11 who were undergoing antiretroviral therapy, 10 who were not undergoing therapy and 10 HIV-negative adult patients with hyperkeratotic oral mucosal lesions. All of the subjects were as- sessed at the Infectious Disease Center, Faculty of Dentistry, Central University of Venezuela, and were clinically examined to detect oral mucosal lesions with the conirmed histopathologic diagnosis. Nested-PCR was used to determine the EBV infection and the latent membrane protein-1 (LMP-1) expression by immunohistochemistry. Results: Of the subjects, 16/21 (76%) of the HIV+/AIDS patients tested positive for EBV, whereas 5/10 (50%) of the HIV-negative subjects tested positive for EBV. Conclusions: In the present study, a higher EBV prevalence was observed in HIV-positive patients when compared to HIV-negative patients without oral hairy leukoplakia, con- irming the etiologic role in this entity. The LMP-1 in OHL patients who were both HIV+ and EBV+ was highly expressed (60%) at the epithelial basal cells. No association between the alcohol and tobacco consumption was observed among the EBV-positive cases. Key words: Oral hairy leukoplakia, human immunodeiciency virus, Epstein Barr virus, latent membrane pro- tein. Article Number: 2613 http://www.medicinaoral.com/ © Medicina Oral S. L. C.I.F. B 96689336 - pISSN 1698-4447 - eISSN: 1698-6946 eMail: medicina@medicinaoral.com Indexed in: -SCI EXPANDED -JOURNAL CITATION REPORTS -Index Medicus / MEDLINE / PubMed -EMBASE, Excerpta Medica -SCOPUS -Indice Médico Español