Arch Microbiol Immunology 2017; 1 (4): 127-132 DOI: 10.26502/ami.93650016 Archives of Microbiology & Immunology Vol. 1 No. 4 - Dec 2017. 127 Research Article Sero-Prevalence of Parvovirus B 19 Infection Among HIV Positive Patients Attending Tertiary Care Hospital in Central India Bhushan Shrikhande 1 , Meena Mishra 1 , Mohiuddin Qazi 1 , Arvind Kurhade 2* , Chandrashekhar Unakal 2 , Geeta Kurhade 3 , Rajendra Surpam 1 , Vyankatesh Katkar 1 1 Department of Microbiology, Government Medical College, Nagpur, India 2 Department of Paraclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago 3 Department of Pre-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago * Corresponding Author: Arvind Kurhade, Department of Paraclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine Campus, Trinidad and Tobago, Email: Arvind.kurhade@sta.uwi.edu; dr_kurhade@rediffmail.com Received: 12 October 2017; Accepted: 28 October 2017; Published: 06 November 2017 Abstract Objective: The aim of this study was to assess sero-prevelance of Parvovirus B19 among HIV positive patients attending tertiary care hospital from central India. Methods: This was a hospital based cross sectional study conducted from June 2014 to September 2014. The study population includes 100 HIV positive patients attending ART Clinic with signs and symptoms suggestive of anemia and hemoglobin <10g/dL were included. Blood samples were collected and the sera obtained after centrifugation of clotted samples were stored at -20ºC. Enzyme-linked immunesorbent assay was used to detect IgG and IgM antibodies. CD4 + count estimation of blood samples was done by Flow cytometry. The demographic information’s were collected from patients using structured questionnaire prior to sample collection. Institutional Ethical committee approval has been taken prior to conduct this study. The consent to collect the study data was also obtained from the patients. The data were analyzed using statistical software Epi Info 7. Results: The mean age of study subjects was 37.44±9.80 years and 55 (55%) were males and 45 (45%) were females. IgG and IgM antibody test showed, 6 (6%) and 11 (11%) cases were positive for anti-parvovirus IgM and