E-Anveshan : Multidisciplinary Journal of Sarojini Naidu Govt. Girls P. G. (Auto.) College, Bpl. ISSN – 2395-5783 Vol. 02, Issue 03 January 2016 45 An Empirical Technique to Resolve Banding Sequences in Avian Chromosomes Dr. H. K. GARG Professor of Zoology Sarojini Naidu Govt. Girls Post-Graduate (Autonomous) College Shivaji Nagar, BHOPAL – 462 006 (India) Abstract :- Banding techniques are useful for marking specific regions of chromosomes, which otherwise exhibit morphological resemblances to a certain extent. C- banding, commonly called constitutive heterochromatin, is frequently used as an approach to resolve variations in terms of amount and distribution of heterochromatin among phylogenetically unrelated species. Another pattern i.e. G-banding is believed to mark the regions rich in guanine and cytosine that replicate DNA early in the synthesis phase of cell cycle. In the present communication, a simple technique has been described, to demonstrate banding sequences, conveniently by stressing chromosomal squash with an alkali, followed by saline treatment (C-band). G-bands can be obtained by exposing metaphase plates to trypsin for a controlled time period before staining with Giemsa-buffer solution. Certain measures have been taken to overcome difficulties, that generally arise, in demonstration of chromosomal bands.