Toxicity Test of Liquid Waste from Printing Industry: Before and after Filtration with Zeolite on Common Carp (Cyprinus Carpio L) Yuli Pratiwi * , Sri Sunarsih, Istiqomah Shariati Zamani Depart. of Environmental Engineering, Faculty of Applied Science, Institute Science & Technology, Akprind, Indonesia The liquid waste from printing industries contains harmful chemicals, such as a remaining material for the screen afdruk, a residual of paint stencil, and a waste of textile washing process. This study aims to determine the quality and toxicity of waste silk screening against 50% Lethal Concentration (LC50) observations of 0-96 hours on common carp, before and after filtered by the zeolite. Results wastewater filtration stencil is then compared with the quality standards of Other Activities Standard. The standard is issued by Yogyakarta Governor on Regulation No.7 year of 2010, The comparison is based on the parameters of pH, COD, BOD, SS. The sample of liquid waste is taken from household printing industry which is located at Ponggok, Trimulyo, Jetis, Bantul, Yogyakarta. The research was conducted in three stages: (a) the stage of acclimatization of common carp; (b) toxicity test prior and after filtration using zeolite, namely a preliminary test and the actual test; (c) waste treatment process using zeolite filtration with 0-50 cm thick. The results show that the optimum thickness of zeolite used for filtration is 50 cm, with improvements pH of 33.4%; COD of 46.00%; BOD of 45.98%; and SS of 52.39%. According to the regilation, the value of pH and BOD are within the permissible value, but the COD and SS is still above the standard. LC50 96 hours prior to the filtration on common carp is at a concentration of 5.66%, whereas after filtration the concentration is at 13.17%. Efficiency of the improvements based on LC50 96 hours is 64.69%. Keywords: Liquid Printing Waste, Toxicity Test, Zeolite, LC50, Common Carp. 1. INTRODUCTION Printing services, one of home industries, is developing rapidly in Yogyakarta. This means that the services supports tourism in Yogyakarta which is one of the destinations for travelers. Several unique souvenirs found in Yogyakarta are made by the printing industries. The existence of screen printing industry is generally still in a household scale, resulting in a lack of supervision of the handling of waste generated. Liquid waste printing can contaminate the environment if improperly handled. The waste contains harmful chemicals, such as remaining photoxol (afdruk making material on the screen), the rest of the rubber (printing paint), as well as the waste of textile washing In order to determine the toxic effects of printing waste water, it is necessary to test the living being with the calculation of Lethal Concentration (LC50). Test animals usually used for this purpose is a common carp (Cyprinus carpio L.), because of the sensitivity on aquatic environment changes [1], [3], [8]. Toxicity test includes acute and chronic toxicity tests which are used to evaluate toxic concentration [1], [3] , in this case the waste water and the exposure time that can cause toxic effects on common carp (Cyprinus carpio L). To eliminate the toxic effects of the waste, it is required waste treatment process which is simple and easy to apply by means of adsorption technique using zeolites. Results of the treatment is projected to fulfil the environmental standards. Zeolite is a combination of crystalline hydrated aluminosilicate which contains geo- alkali or alkaline cation, zeolite can absorb gas or liquid and able to separate molecules based on the polarity [2], [7]. The purposes of the work are: (a) determine the quality of printing waste water before and after filtering process using zeolite; (b) define the LC50 0-96 hour printing waste water to Cyprinus carpio L) before and after zeolite filtering; (c) optimize the thickness of zeolite for the filtration based on the parameters of pH, COD, BOD, SS; (d) compare the quality of the filtered waste water with the Standard Quality of Industrial Activities Standard based on pH, COD, BOD, and SS which is found in Yogyakarta governor regulation No.7, 2010 [5]. 2. METHODS Waste water sample is taken from household printing industry which is located at Ponggok, Trimulyo, Jetis, Bantul, Yogyakarta. Meanwhile, the test fish used is Cyprinus carpio L. The sample is taken from waste water of printing and screen cleaning before disposing into the final sewer using the composite method. This research was conducted in four stages: (a) the stage of acclimatization of common carp (Cyprinus carpio L); (b) toxicity test prior to filtration using zeolite, namely a preliminary test and the actual test; (c). waste treatment process using zeolite filtration with 0-50 cm thick; (d) toxicity test after the filtration, which includes a preliminary test and the actual test. International Journal of Engineering Research & Technology (IJERT) ISSN: 2278-0181 http://www.ijert.org IJERTV6IS030137 (This work is licensed under a Creative Commons Attribution 4.0 International License.) Published by : www.ijert.org Vol. 6 Issue 03, March-2017 110