International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-4, Issue-2, February- 2018] Page | 63 Evaluation of Total Petroleum Hydrocarbon (TPH) in Sediments and Aquatic macrophytes in the River Nun, Amasoma Axises, Niger Delta, Nigeria. Alagoa, K.J 1* , Godwin, J 2 , Daworiye, P.S 3 , Ipiteikumoh, B 4 1 Department of Biological Sciences, Niger Delta University, Amasoma, Bayelsa State. 2 Department of Chemical Sciences, Niger Delta University, Amasoma, Bayelsa State. 3 Department of Biological Sciences, Isaac Jasper Boro College of Education, Sagbama, Bayelsa State 4 Department of Mathematics, Isaac Jasper Boro College of Education, Sagbama, Bayelsa State. Abstract— The Total Petroleum Hydrocarbon (TPH) in the bottom sediments and aquatic macrophytes of the River Nun at AmasomaAxises was investigated in December, 2017. This was done to establish the existing levels of TPH in the River in order to ascertain the degree of its threat to the environment. Benthic samples were collected close to the shore with the aid of a hand trowel and put in aluminum foils, while macrophyte samples were collected by hand pulling and transported in plastic bags for laboratory analysis. Data were subjected to statistical analysis using the Microsoft Excell ® tool pack. Regression analysis was employed in order to determine the correlation between TPH in bottom sediments with that in plant tissues (leaf, stem and root). Analysis of variance was employed at the 95% confidence level to determine the degree of significance in interaction of TPH between sediments and macrophyte tissues and between macrophyte tissues (leaf, stem, and root). Duncan multiple range test was use to compare means. The bioaccumulation factor (BAF) was calculated for TPH in order to estimate the absorption rate of TPH between sediments and plant tissues (leaf, stem and root). Results indicate that TPH were recorded in sediments and plant tissues. TPH concentrations were greater in plant tissues than in sediments. Concentration of TPH showed concentrations indicating that root> stem> leaf in most stations. The relationships between the TPH attributes indicated strong association between Leaves and stem (r 2 = 0.92). Strong negative association was also observed between sediment and stem (r 2 = -0.83) while sediment and leaves (r 2 = -0.64) had moderate negative relationship. It can be concluded that the River Nun at AmasomaAxises is mildly polluted due to land based activities and therefore there is a need to enact water use regulatory ordinances to protect its ecology. Sediment organisms and plants are vital links to man in the food chain. This may portend danger in the future. Keywords— Total Petroleum Hydrocarbon, Sediments, Macrophytes, Nun River. Amasoma. I. INTRODUCTION Total petroleum Hydrocarbon (TPH) is a term used for any mixture of hydrocarbons found in crude oil. They are several hundred of these compounds, but not all occur in any one sample. There are so many different chemicals in crude oil and other chemical products for instance that it is not practical to measure each one separately. Therefore the measurement of TPH becomes most apt, as it gives a definitive picture of the whole. The contamination of aquatic ecosystems with TPH is a disturbing reality. The majority of TPH entering aquatic environments remain close to sites of deposition, suggesting that lakes, rivers, estuaries and coastal environments near centers of human population are the primary repositories of the aquatic TPH [1]. Discharges from urban catchments may carry with them sediments, nutrients, heavy metals, pesticides, oils and hydrocarbons and solid pollutants such as litter [2]. Also, Industrial discharges also carry significant TPH and thermal load to receiving environments [3]. TPH sources of pollution of the aquatic environment are so diverse that no single source can be held culpable. Sadly, the accumulation of TPH in an aquatic environment has direct consequences to man and to the ecosystem due to the intrigues of food chain [4]. This is as a result their easy affinity for bottom sediments and plant tissues. This may imply serious health implications from bioaccumulation and bio-magnifications in living organisms. Therefore the fate of TPH introduced by human activities into aquatic ecosystems have become the subject of wide spread concern, since beyond the tolerable limits they become toxic [5, 6].The measurement of TPH in the aquatic ecosystem is a universally accepted practice for determining the pollution status and integrity of water bodies. The River Nun at the Amasoma axis is a fresh water body and an appendage that lies along the 160km flow course of the River from its flow origins of the River Niger. It is the main live-wire of the Amasoma people as it provides their source for