4/5/2018 A Review of PCR-Based DNA Fingerprinting... (PDF Download Available) https://www.researchgate.net/publication/265275300_A_Review_of_PCR-Based_DNA_Fingerprinting_Using_Arbitrary_Primers_in_Tropical_Ornamental_Fishes_ Article (PDF Available) · January 2011 with 104 Reads A Review of PCR-Based DNA Fingerprinting Using Arbitrary Primers in Tropical Ornamental Fishes of South- East Asia Abstract DNA  ngerprinting has assumed an important role in aquaculture and  sheries management. Molecular genetic markers have been developed into powerful tools to analyze genetic diversity and relationships among different aquatic species. Besides the varied techniques using polymorphic DNA markers, Random Ampli ed Polymorphic DNA (RAPD) markers generated by Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) have become ubiquitous and essential in aquaculture genetics. Single 9-20 base oligonucleotide primers with G+C contents ranging between 40-80% were randomly chosen for PCR to generate useful genomic  ngerprints. DNA ampli cation products were resolved by denaturing polyacrylamide gel electrophoresis (dSDS-PAGE). Subsequent detection by silver staining could easily allow the visualization of fragments below 3.5 kb. The spectrum of ampli ed products varied with each primer-template combination but was consistent and reproducible under similar PCR conditions. The primers could detect polymorphisms in the absence of speci c nucleotide sequence information of the  shes. Such DNA polymorphisms are especially useful for genotyping, taxonomic identi cation, population studies and genetic mapping. This approach is also applicable for assessing inbreeding levels and population structure within wild and cultivated  sh and shrimp stocks. Here, we review the development, applications and advances of AP-PCR in elucidating DNA polymorphisms in tropical ornamental  shes. Genetic differences among species and strains, sexes, species-and phenotype-speci c DNA polymorphisms, and applications of DNA marker-based approaches in  sh breeding and management programmes are discussed. Full-text (PDF) Available from: Gideon Khoo, Oct 10, 2014 Cite this publication 18.17 · Universiti Tunku Abdul Rahman Gideon Khoo 36.07 · National University of Singapore Tit Meng Lim Violet P E Phang Discover the world's research 14+ million members 100+ million publications 700k+ research projects Join for free Download full-text PDF or Search for publications, researchers, or questions Discover by subject area Join for free Log in See all › 4 Citations See all › 104 References Download citation Share Download full-text PDF