Global Advanced Research Journal of Medicine and Medical Sciences (ISSN: 2315-5159) Vol. 5(5) pp. 143-149, May, 2016 Available online http://garj.org/garjmms Copyright © 2016 Global Advanced Research Journals Full Length Research Paper Probiotic Potency and Molecular Identification of Lactic Acid Bacteria Isolated from Bali Cattle’s Colon, Indonesia I Wayan Suardana 1 *, Annisa Putri Cahyani 2 and Komang Januartha Putra Pinatih 3 1 Department of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar. Jl. PB. Sudirman Denpasar-Bali. 80232 Indonesia. 2 Undergraduate Student of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar. Jl. PB. Sudirman Denpasar-Bali. 80232 Indonesia. 3 Department of Microbiology, Faculty of Medicine, Udayana University Denpasar. Jl. PB. Sudirman Denpasar-Bali. 80232 Indonesia. Accepted 19 May, 2016 Lactic acid bacteria (LAB) is a group of bacteria which able to produce lactic acid as a main product. LAB normally live in the digestive tract of humans and animals, and generally use as probiotics. On the other hand, Bali cattle are known has highly adaption to poor quality of feed so that it is suggested that specific types of LAB can be found. The aims of this study was to determine the probiotics producing by LAB isolates 18A isolated from colon of Bali cattle as well as to identify of isolate by analysis of the 16S rRNA gene sequence. Probiotic potency analysis was performed by cultivation of LAB isolates 18A on the Man Rogosa Sharpe Broth (MRSB) medium, continued by Gram staining and catalase test. Antimicrobial activities of isolate were done against pathogenic bacteria (E. coli KL 48 (2) and S. aureus). Furthermore, genetic analysis was initiated by DNA isolation, amplification of the 16S rRNA gene, and sequencing. The result showed that LAB isolate 18A has inhibitory efficacy against gastrointestinal pathogens such as Escherichia coli KL 48 (2) and Staphylococcus aureus by 18,8% and 28,06% respectively. Moreover, genetic analysis showed the LAB isolate 18A as Enterococcus durans with the similarity level of 99%, and a value of 100 bootstrapping. Keywords: Lactic acid bacteria, 16S rRNA gene, antimicrobial activity, Bali cattle’colon. INTRODUCTION Lactic acid bacteria (LAB) is a group of bacteria which able to produce lactic acid as the main products through homofermentation and heterofermentation process. The *Corresponding Author E-mail: iwayansuardana22@yahoo.com, wayan_suardana@unud.ac.id; Mobile: +62 81338621615; Tlp/Fax : +62(361) 223791 acidification caused by enzymatic processes of the growing LAB affect the taste, texture and durability of a fermented foods (Klaenhammer et al., 2002). Lactic acid bacteria can be found in pickled fruits and vegetables (Bae et al., 2006; Chambel et al., 2006; Nyanga et al., 2007; Duangjitcharon et al., 2007), fruit drinks (Plessis et al., 2004), sausage (Ammor et al., 2005), rumen fluid of Bali cattle (Suardana et al., 2007), milk (Imen et al., 2016) and wheat, rice, cassava (Reddy et al., 2008).