www.ejbps.com 1083 Kandil et al. European Journal of Biomedical and Pharmaceutical Sciences RELATIONSHIP BETWEEN INSULIN-LIKE GROWTH FACTOR-1 AND CYSTATIN-C IN PATIENTS WITH CHRONIC KIDNEY DISEASE Yasser I. Kandil 1* , Ahmed I. Abulsoud 2 , Tarek M. Salman 3 , Maha M. Akl 4 , Ragaa R. Mohamed 5 1*,2,3 Biochemistry Department, Faculty of Pharmacy, Al-Azhar University-Cairo, Egypt. 4 Pathology Department, Faculty of Medicine Al-Azhar University Cairo, Egypt. 5 Internal Medicine Department, Faculty of Medicine Al-Azhar University, Cairo, Egypt. Article Received on 27/06/2015 Article Revised on 16/07/2015 Article Accepted on 10/08/2015 ABSTRACT Background: Chronic kidney disease (CKD) is a life-threatening condition characterized by progressive and irreversible loss of renal function. Due to its high prevalence and associated mortality, CKD is an important human and social burden. Cystatin-C, an endogenous protein produced by nucleated cells, is a promising biomarker to estimate the glomerular filtration. Insulin-like growth factor-1 (IGF-1) has been associated different disorders that affect renal functions, it is suggested that increasing levels of serum IGF-1 will be positively associated with CKD and that IGF-1 might be a predictor of CKD. Objective: To examine the relationship between serum levels of IGF-1 and cystatin-C in different stages of CKD. Patients and Methods: seventy patients with CKD and twelve healthy controls were included in this study. The patients were subclassified into five groups according to their CKD stages based on their eGFR. Urea, creatinine, albumin, sodium, potassium, calcium, and phosphorus were measured spectrophotometerically. Also, serum cystatin-C was measured by ELISA and IGF-1 by chemiluminescence technique. Results: a highly significant increase in serum cystatin-C level in all stages of CKD when compared to control group. There was a highly significant increase in serum IGF-1 level between patients with CKD-4 and CKD-5 when compared to the control group where or the other stages of CKD. Finally, IGF-1 showed a highly positive correlation to cystatin-C. Conclusion: serum levels of both cystatin-C and IGF-1 are increased among the different stages of CKD and they are positively correlated. European Journal of Biomedical AND Pharmaceutical sciences http://www.ejbps.com ISSN 2349-8870 Volume: 2 Issue: 4 1083-1093 Year: 2015 Research Article ejbps, 2015, Volume 2, Issue 4, 1083-1093. SJIF Impact Factor 2.062 *Correspondence for Author Yasser I. Kandil Biochemistry Department, Faculty of Pharmacy, Al- Azhar University Cairo, Egypt.