~ 1212 ~ Journal of Entomology and Zoology Studies 2018; 6(2): 1212-1216 E-ISSN: 2320-7078 P-ISSN: 2349-6800 JEZS 2018; 6(2): 1212-1216 © 2018 JEZS Received: 20-01-2018 Accepted: 23-02-2018 NM Bhojane Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India RS Ingole Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India SW Hajare Departments of Pharmacology and Toxicology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India SV Kuralkar Department of Animal Genetics and Breeding, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India SJ Manwar Department of Poultry Sciences, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India SP Waghmare Teaching Veterinary Clinical Complex, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India Correspondence RS Ingole Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Krishi Nagar, Akola, Maharashtra, India Individual and combined toxicity effect of indoxacarb and glyphosate on general performance and hematological parameters in Japanese quails NM Bhojane, RS Ingole, SW Hajare, SV Kuralkar, SJ Manwar and SP Waghmare Abstract The present study was conducted to assess the general performance, clinical and the hematological observations in sub-acute toxicity of indoxicarb and glyphosate in Japanese quails. Two week old, 252 Japanese quails after one week acclimatization were divided into seven equal groups and group C served as control, whereas group T1 was fed with feed + indoxacarb @ 30 mg/kg of feed, group T2 was fed with feed + indoxacarb @ 60 mg/kg of feed, group T3 was fed with feed + glyphosate @ 250 mg/kg of feed, group T4 was fed with feed +glyphosate @ 500 mg/kg of feed, group T5 was fed with feed + indoxacarb@ 30 mg/kg of feed +glyphosate @500 mg/kg of feed and group T6 was fed with feed +indoxacarb @ 60 mg/kg of feed + glyphosate @ 250 mg/kg of feed for a period of four weeks. The clinical signs observed in combination group were more prominent compared to birds fed with individual indoxacarb or glyphosate at higher doses. Decreases in the body weight in quails was observed in all treatment groups but glyphosate causes significant decrease in body weight compared to indoxacarb. There was marginal reduction in feed intake in quails receiving high dose of indoxacorb and glyphosate alone and combination treated groups. The non-significant alteration in hematological paramenters indicated low toxic minimal adverse toxicity effect of indoxacarb or glyphosate either singly or in combination in Japanese quails for a period of 28 days. Keywords: glyphosate, hematology, indoxacarb, Japanese quails, toxicity Introduction Indoxacarb (S)-methyl 7-chloro-2, 5- dihydro-2-[(methoxycarbonyl) [4 (trifluoromethoxy) henyl] amino]-carbonyl] indeno [1, 2-e] [1, 3, 4] oxadiazine-4a-(3H)-carboxylate (C22H17ClF3 N3O7) is a new insecticide of class oxadiazine having strong activity against lepidopteron pests of fruits, vegetables, corn, tree cotton, peanut, soybean, alfalfa and other crops recommends it`s wide use in agriculture and horticulture [1] . The activated metabolite of indoxacarb has a unique mode of action by blocking of sodium channels of nerve cells, resulting in paralysis and death of the target pest species [2] . The neuronal cholinergic nicotinic receptors are the one of the primary target sites of the insecticide in mammals [3] . Glyphosate (N-[phosphonomethyl]-glycine, H203P-CH2-NH-CH2-C02H) is a unique broad- spectrum post emergence herbicide being used worldwide for the control of terrestrial weeds [4] . It was primarily developed for noncrop agriculture use; however, it has also been used in connection with insecticide tolerant crop varieties and in forestry management practices for site preparation and herbaceous weed control [5] . Generally, glyphosate is slightly toxic to mammals and fish, but it may have an impact on poultry industry through feed residual effect. A higher concentration of glyphosate was detected when glyphosate was sprayed several times during the crop cycle, and when treatments approached the flowering stage [6] . The use of insecticide in combinations for agricultural pest control and public health is increasing day by day due to the challenges posed by insecticide resistance to the existing chemicals. Past research in agriculture sciences showed excellent effects of mixing of glyphosate with numerous insecticides (including indoxacarb) in various agricultural crops; however no information is available on toxicopathology of combined effect of indoxacarb and glyphosate in avian, animals and humans. Therefore, there is a need to study toxicopathology of indoxacarb and glyphosate to evaluate health hazards and toxicity associated with dietary