a SciTechnol journal Research Article Gad et al., J Plant Physiol Pathol 2018, 6:1 DOI: 10.4172/2329-955X.1000176 Journal of Plant Physiology & Pathology All articles published in Journal of Plant Physiology & Pathology are the property of SciTechnol, and is protected by copyright laws. Copyright © 2018, SciTechnol, All Rights Reserved. International Publisher of Science, Technology and Medicine Eficacy of Camphor and Casuarina Leaf Powder or Water Extracts on Meloidogyne Incognita Infecting Tomato Plant under Green House Condition Gad SB 1 *, Osman MA 2 and Sergany MI 3 Abstract A pot experiment was carried out to evaluate the impact of camphor and Casuarina dry leave powders or water extracts as single or mixed application with nematicide on tomato plant infected with of M. incognita under greenhouse conditions. The Triple application of ⅓ Casuarina and ⅓ camphor water extract (5 ml each) + ⅓ oxamyl (0.1 g) was signiicantly (P ≤ 0.05) exceed other materials in the increment of total plant growth parameters and recorded high values of nematode reduction criteria that were amounted to 91.5, 92.53 and 94.81%, respectively, comparing to nematode alone. The application of dual ½ Camphor water extract and ½ oxamyl at halve doses each beset other binary treatments in the increase percentage values of such plant growth features and nematode parameters too. The nematode pesticide, oxamyl recorded a signiicant (P ≤ 0.05) increment in plant length (51.86%), shoot fresh weight (36.79%) and shoot dry weight (64.31 %) respectively and recorded a lowest reproduction factor (RF) with value 0.16 as compared with compared to those received nematode alone (2.43). Keywords M. incognita; Camphor; Casuarina; Nematode; Water extract; Dry leave powder *Corresponding author: S.B. Gad, Nematology Research Unit, Agric. Zool. Department, Faculty of Agriculture, Mansoura University, Dk. Egypt, Tel: 01009142087; E-mail: Samirborham@mans.edu.eg Received: September 26, 2017 Accepted: Janaury 06, 2018 Published: January 12, 2018 Introduction he southern root-knot nematode, Meloidogyne incognita, is one of the mainly economically injurious genera of plant-parasitic nematodes in Egypt [1]. Root-knot nematodes are distributed global as well as and are obligate parasites of the roots of thousands of plant species, and woody plants, ornamental and vegetable crops. he root- knot nematode predisposes the plants by other pathogens. But no speciic control measures have yet been adopted to save economic plant from the root-knot disease. Chemical control of root knot nematode is costly and hazardous to agro-ecosystem, environment and human health. Many plants possessing inhibition efects like pesticides properties [2]. Several plant species belong to 57 families known to have nematicidal properties [3]. Nematicidal phyto- chemicals are normally safe for the eco-system and human health [4]. hese compounds might have efects like to the artiicial organic compounds. Camphor plant (Cinnamomum camphora) is believed to be toxic to insects and an antimicrobial compounds. Ancient Egyptians used camphor oil in mummiication. Camphor leaves contains nematicidal components such as cineol, pinene, terpineol, menthol, and thymol [5]. Casuarina plant, (Casuarina equisetifolia) is a source of biologically active antioxidants compounds i.e. catechin, ellagic acid, gallic acid, quercetin and lupeol. Casuarina has showed antimicrobial activity [6]. It was thought important to study the efect of certain common materials, camphor and casuarina plant leave powders or water extracts on the incidence of root-knot nematode M. incognita infecting tomato plants under green house conditions. Materials and Methods Plant powder and water extract preparation Leaves of camphor (Cinnamomum camphora) and Casuarina (Casuarina equisetifolia) were collected and botanical deined. he obtained leaves were sun dried then grounded by belender. plant powders were applied at the rate of 15 g/pot. Water plant extract of such plant were prepared by using quantity of 25 g of fresh green leaves which mixed with 100 ml distilled water within petri desh. One day Later, iltered and all the iltrates were collected separately for use as extracts in the pot experiments and were applied at the rate of 15 ml/pot. Nematicide Oxamyl (10% G): Methyl-N,N-dimethyl-N[(methyl) carbamyloxy]-1-thioxamidate was used at the bespoke rate (0.3 g/ pot). Experimental design he recent study was conducted under greenhouse conditions at (29 ± 5˚C) to evaluate the nematicidal activities of two selective medicinal and aromatic plant species against M. incognita and the resulting efect on tomato plants Hybrid F1 at Nematology Research Unit, Agricultural zoology Department, Mansoura Unieristy. Sixty plastic pots (15 cm-d) were packed with admixture of one kg autoclaved sandy loamy soil (1:1, v/v). Each pot was individually planted by one tomato seedling 30 days old at the time of transplanting. One week later, pots were each inoculated with 2000 second stage of juveniles (J 2 ) of M. incognita. To obtain nematode juveniles inoculation, juveniles (J 2 ) were extracted from soil of coleus plants by sieving and modified Baermann technique [7] counted in a Hawksely counting slide under 10 × magnifications then calculated for each 1 ml of the nematode suspension and recorded for preparing nematode inoculation of this work. The tested pots supplemented with dried powder of two botanicals namley camphor and Casuarina dried leave powder or water extracts singly or combined with oxamyl as double or triples application. Five pots were treated with oxamyl (10% G) at the rate of 0.3 g/pot. However, five pots were received nematode alone. Another five pots were served as untreated uninoculated plants (plant check). There were thirteen treatments with 5 replications as following: