Alytes, 2018, 35 (1–4): 1–11. Phytotelmata selection by anurans and implications for their conservation at Las Tablas Protected Zone, Costa Rica Diego A. GÓMEZ-HOYOS 1, 2, * , Sergio ESCOBAR-LASSO 3 , Rocío SEISDEDOS-DE-VERGARA 1 , Juan G. ABARCA 4 , Jan SCHIPPER 1, 5 , Steven M. WHITFIELD 6 , Gilbert ALVARADO 7, 8 & José F. GONZÁLEZ-MAYA 1 1 ProCAT Internacional / Sierra to Sea Institute Costa Rica, Las Alturas, Coto Brus, Puntarenas, Costa Rica. 2 Grupo de Estudio en Herpetología de la Universidad del Quindío (GHUQ), Armenia, Quindío, Colombia. 3 Fundación RANA (Restauración de Ambientes Neotropicales Alterados), Colombia. 4 Centro de Investigación en Estructuras Microscópicas (CIEMIC), Universidad de Costa Rica, Ciudad de la Investigación, San José, Costa Rica. 5 Arizona Center for Nature Conservation – Phoenix Zoo, Phoenix, AZ, USA. 6 Zoo Miami, Conservation and Research Department, Miami Florida, USA. 7 Laboratory of Comparative Wildlife Pathology, School of Veterinary Medicine and Animal Sciences, University of São Paulo. São Paulo, Brasil. 8 Escuela de Biología, Universidad de Costa Rica, Campus Rodrigo Facio, San Pedro de Montes de Oca, Costa Rica. * Corresponding author <dgomez@procat-conservation.org>. Phytotelmata are microhabitats used for shelter by many anuran species. Understanding anuran-microhabitat relation- ship is important for their conservation as it could notably affect host-pathogen interactions (i.e. chytrid fungus- amphibians), and could help us to understand their habitat disturbance responses. Herein, we studied spatial and temporal patterns of phytotelmata selection by anurans at Las Tablas Protected Zone, Costa Rica. Also, we assessed the relationship between phytotelmata use and selection with habitat perturbation and Bd fungus, as well as its implications for anuran conservation at the specific locality especially for the critically endangered Atelopus varius. We used the standardized Manly index to estimate proportion between use and availability of Araceae and Bromeliaceae in late-succes- sional forest and perturbed zones. We also evaluated the presence of the chytrid fungus by standard PCR techniques. We found a spatial and temporal pattern in phytotelmata use and selection that should be considered for anuran conservation at