Vol. 73 | No. 12/1 | Dec 2017 DOI: 10.21506/j.ponte.2017.12.21 International Journal of Sciences and Research 314 A Simple Tool for Identifying Outer Shape of White Blood Cell Based on Image Processing Techniques in Order to Develop Health Facilities in Developing Countries Retno Supriyanti Departement of Electrical Engineering, Jenderal Soedirman University, Indonesia, Email: retno_supriyanti@unsoed.ac.id Mahmud M Afif *, Ikhsani T. Hasan * , Yogi Ramadhani*, Wahyu Siswandari ** * Departement of Electrical Engineering, Jenderal Soedirman University ** Departement of Medical, Jenderal Soedirman University Abstract The shape and size of white blood cells in the blood are often used as indicators of the white blood cell abnormalities. Meanwhile, the diameter of the white blood cells can be used for cell classification and identification if there are abnormalities of white blood cells. During the identification of white blood cells is done manually by medical personnel with the help hemocytometer that calculation results are still dependent on the observer subjectivity. Another way is by hematology analyzer that has a more precise accuracy but at a costly price. Digital image processing is a method of processing an object in a digital image automatically. In this case, it can be an alternative calculation of the s and diameter of blood cells. In this research, the white blood cells identified using Active Contour methods. The results show that this method successfully detects the contours of 200 white blood cells images in a relatively short time. Keywords: White blood cell, Diameter calculation, Hemocytometer, HSV method, Color segmentation Introduction White blood cells or commonly called leukocytes are cells of the immune system that protects the body against infectious disease and foreign particles. All leukocytes are produced and derived from multipotent cells in the bone marrow known as a hematopoietic stem cell. Leukocytes can be found throughout the body, including blood and lymph system. The number of white blood cells in the blood are often used as indicators of the white blood cell abnormalities. If the number of white blood cells is too little, the body will have problems in the fight against infection. In contrast, the number of white blood cells is too much can indicate leukemia, certain infections, or conditions like measles or whooping cough. While the diameter of the white blood cells is useful to classify the white blood cells, as well as indicating abnormalities in the white blood cells if the diameter of abnormal blood cells [1]. In general, the white blood cell count is done manually using a microscope and hemocytometer by a physician or medical personnel health laboratories. But it does have the disadvantage that the subjectivity of the human is very high, which might be due to lack of concentration or misuse of the equipment itself. In addition, it also time consuming. Another way is to use a hematology analyzer. This tool can calculate simultaneously measuring the diameter of blood cells automatically, but the expensive price makes this tool still rarely used. Therefore, it is need other methods as an alternative in the calculation of the number and diameter of blood cells automatically and affordable.