The International Journal of Innovative Approaches in Agricultural Research Volume 1 Issue 1, 2017 51 Nyam D.D. 1 University of Jos, Nigeria Kwon-Ndung E.H. 2 Federal University Lafia, Nigeria Wuyep A. P. 3 University of Jos, Nigeria Abstract The evaluation of the molecular diversity of Acha (Digitaria sp.) was carried out using Microsatelite primer combinations specially designed forDigitaria exilis. The amplifiedmicrosatellite fragments were also used in the other species of Digitaria namely; Digitaria barbinodis and Digitaria iburua.DNA extraction was carried and Agarose Gel Electrophoresis was conducted on the restricted amplified DNA extracts using microsatellite primers developed for Digitaria exilis. RFLPs clearly suggest a distinct separation of the three species of Acha at the molecular level ( D. iburua, D. exilis and D. barbinodis) demonstrating the extent of their genetic differences at the DNA level. Keywords: Gel, electrophoresis, RFLP, Acha 1 Department of Plant Science and Technology, University of Jos, Nigeria 2 Department of Botany, Federal University Lafia, Nigeria Correspondence: kwon_ndung@yahoo.com 3 Department of Plant Science and Technology, University of Jos, Nigeria