IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 17, Issue 1 Ver. 17 January. (2018), PP 36-43 www.iosrjournals.org DOI: 10.9790/0853-1701173643 www.iosrjournals.org 36 | Page Antioxidant status, Oxidative stress and 5’-Nucleotidase activity in alcoholic and drug induced cirrhotic patients Md. Abu Nasar 1 , Tarannum Fatima Subhani 2 , Rajiv Ranjan Sinha 1 1 Department of Biochemistry, Nalanda Medical College,Patna,India 2 Department of Biochemistry, Al Falah School of Medical Sciences and Research Centre, Faridabad, Haryana, India *Corresponding Author: Dr Tarannum Fatima Subhani Abstract Background: The present study was undertaken to estimate the 5’ -nucleotidase enzyme in drug induced liver cirrhosis and alcoholics. The oxidative stress, antioxidants and their correlation with 5’ -nucleotidase was also included in this study. Methods: The blood samples of 25 subjects (age and sex matched) each from group I (control), group II (alcoholic) and group III (drug induced cirrhotic) was taken and centrifuged for separation of plasma for analysis of 5’-nucleotidase. The separated cells were washed thrice with 0.9 % w/v cold normal saline and used for the analysis of glutathione, malondialdehyde and superoxide dismutase. Results: The activity of serum 5’- nucleotidase was significantly increased in both drug induced cirrhotics and alcoholics. The levels of malondialdehyde were also significantly increased in both drug induced cirrhotic and alcoholic patients. The levels of glutathion and superoxide dismutase were significantly decreased in both drug induced cirrhotics and alcoholics. Conclusions: From these findings it was concluded that the activity of serum 5’ - nucleotidase rises consistently in drug induced cirrhotic and alcoholics according to the extent of liver damage, hepatobiliary damage, and biliary stasis and can be a useful marker for diagnosis of hepatobiliay disorders. Keywords: Alcoholics; Antioxidant effects; Cirrhotics; 5’- nucleotidase; Oxidative stress. --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: 17-01-2018 Date of acceptance: 03-02-2018 --------------------------------------------------------------------------------------------------------------------------------------- I. Introduction 5‟- nucleotidase (5 NT) is an intrinsic membrane glycoprotein, present as an ectoenzyme in a wide variety of mammalian cells, hydrolyzes 5‟-nucleotides to their corresponding nucleosides (1). Elevations in the 5 NT serum level appear to be restricted to intrahepatic or extrahepatic obstructive disease with no change in other organs disease (2). It is measured as an indicator of liver damage resulting primarily from interference with the secretion of bile (2). Enzyme activity elevates in all diseases including liver cirrhosis, chronic alcoholism, neoplasms of liver and bile ducts, benign biliary disease but reaches its highest level in the presence of biliary stasis (3-4). 5 NT is also a sensitive marker for hepatic metastasis either by itself or in combination with other enzymes. The diagnostic value of 5 NT has been shown to be superior to other liver enzymes, especially in liver metastasis. Raised levels of 5 NT activities are found in 92 % of patient with obstructive jaundice, 70 % of patients with parenchymal liver disease and 81 % of patients with hepatic metastasis (5-6). It is also reported that serum 5 NT is clinically useful for differential diagnosis of hepatobiliary and osseous diseases, the enzyme activity being increased only in hepatobiliary disease (7). Because it is a plentiful primary liver enzyme, the 5 NT may be more readily influenced, by minute areas of obstruction than is the alkaline phosphatase (2, 8). Although determination of either oxidants or antioxidant components alone may give information about the oxidative stress, determination of oxidants along with antioxidants is more useful in this context (9). Oxidative stress has been implicated in liver cirrhosis. Studies suggested that evidence of oxygen free radical is found early in the development of fibrosis and cirrhosis of liver (10). Patients suffering from liver disease either due to drug induced or excessive alcohol intake shows depletion of antioxidants such as glutathione (GSH) and superoxide dismutase (SOD) and increased concentration of product of lipid peroxidation such as MDA (11-19). Depletion of antioxidants such as GSH occurs as a result of decreased production from the diseased liver and consumption of antioxidants due to increased oxidative stress. Depletion of antioxidants renders the cell more susceptible to oxidative stress. Antioxidant and stress related enzymes might be able to determine the degree of liver damage (16-19). Hepatic fibrogenesis in alcoholic liver cirrhosis is an intricate process, which appears to involve a metabolic products of ethanol oxidation; chytochrome P450 induction, enhanced oxidative stress, depletion of