International Journal of Advanced Engineering Research and Science (IJAERS) [Vol-5, Issue-6, Jun- 2018] https://dx.doi.org/10.22161/ijaers.5.6.26 ISSN: 2349-6495(P) | 2456-1908(O) www.ijaers.com Page | 153 Proximate Analysis of Seed Extracts and Methanol Content of Juice of Some Grape Varieties in Turkey Ozcan Baris Citil 1 , Yener Tekeli 2 , Aydin Akin 3 , Fatih Sevgi 4 , Tuba Tekeli 5 1 Department of Veterinary, Karapinar Aydoganlar Vocational School of Higher Education, University of Selcuk, Email: ozcancitil@gmail.com 2 Pharmacy Faculty, Adiyaman University, Turkey Email: yenertekeli@gmail.com 3 Department of Horticulture, Faculty of Agriculture, University of Selcuk,Turkey 4 Department of Medicinal Laboratory, College of Health Care, University of Selcuk, Konya 5 İskenderun Vocational College, Iskenderun Technical University Hatay Abstract— The present study describes the determination and comparison of antibacterial activity, total phenolic content in seeds of seven popular grape cultivars and methanol content of grape juice grown in Turkey. It’s called Muskule, Gokuzum, Razaki, Akuzum, Eksi kara, Siyah pekmezlik and Buzgulu in Turkey. The antibacterial activity was evaluated by the microdilution method and the minimum inhibition concentrations of extracts from determined. The phenolic content was determined using spectrophotometer. Pollution from methyl alcohol, which is extremely harmful for human health was determined using GC. The minimum inhibitory concentration (MIC) values of grape extracts with antibacterial activity ranged from 0,312 to 5 μg/ml. The total phenolic content of grape seed of Ekşi kara was found to be about one-half times as mg GAE/g compare to Büzgülü. The highest methyl alcohol ratios are found in Ekşi kara among juice of grape varieties (12.01 g/μl). Keywords—Vitis vinifera L.; Antibacterial activities; Total phenolic content; Methyl alcohol. I. INTRODUCTION Grape production in our country is a very important fruit species. Seeds of grape varieties contain large amounts of phenolic compounds [1]. These compounds have many favourable effects on human health. For example, human inhibited low-density lipoprotein [2]. Risk of cancer are reduces [3]. The defatted seeds as a rich source of polyphenolic compounds have been largely studied by several research groups [4]. During the chewing, part of the phenolic compounds in grapes are transferred to the human body. Sources of antimicrobial compounds are grape seed extracts [1]. Also, it should be mentioned the importance of phenolics in determining some quality attributes and properties in fresh fruits and vegetables, like the color, texture, taste and flavor. One of the principal roles that have been proposed as part of the actions of phenolics in man is that of an antioxidant [5]. In nature there are a large number of different types of antimicrobial compounds that play an important role in the natural defence of all kinds of living organisms [6].There are high amount linoleic acid in seeed of grape varieties [7]. Linoleic acid consumption may reduce the level of high density lipoprotein cholesterol, increasing the risk for coronary heart disease mortality, increased risk of diabetes, cardiovascular disease and pathophysiological mechanisms impairing insulin activity [8]. Methanol that formaldehyde, and formic acid turned into indicates a toxic effect in the body. Amounts of alcohols depends on grape variety, fermentation conditions, method of distillation and duration of extraction. Due to these compounds is high boiling points during distillation take place in the final product [9]. Studies are announced in alcoholic beverages containing methanol, can cause eye defects, skin cancer and deaths. Methyl alcohol is toxic effect from skin inhaled or absorbed from the gastrointestinal route. Methyl alcohol can cause acidosis, severe abdominal pain, disturbances of consciousness or visual disturbances [10]. For these reasons, grape consumerism is important to know the amount of methanol. Many species of Vitis were studied for especially volatile constituents. Some papers have been referred to volatile compounds in endemic grape samples [11]. Although similar studies on some grape seed cultivars in Turkey, a comparative study on methanol content of grape juice and antibacterial activities, phenolic content of seven popular grape seed cultivars in Turkey has not been reported up to now, according to our knowledge. The present study was carried out to determine and compare methanol content of grape juice and the antibacterial activities, phenolic content of them.