IJSRSET1841310 | Received : 11 Feb 2018 | Accepted : 21 Feb 2018 | January-February-2018 [(4) 1 : 1396-1400 ] © 2018 IJSRSET | Volume 4 | Issue 1 | Print ISSN: 2395-1990 | Online ISSN : 2394-4099 Themed Section : Engineering and Technology 1396 Naked Eye Detection of Thiols by using Pyrene based Chemosensor in Aqueous Medium Milind S. Thakare 1 *, Pravin M. Yeole 2 1 Synthetic Organic Research Lab, Department of Chemistry, Pratap College, Amalner, Maharashtra, India 2 PG Research Lab, Department of Chemistry, R. L. College, Parola, Maharashtra, India ABSTRACT Thiols are well known biological species plays a major physiological role. Sensitive and quantitative sensing thiols is therefore of great significance to public health investigation A new Pyrene based turn-on fluorescent probe based on the Anchoring and Unanchoring strategy for the selective detection of thiol was synthesized. The pyrene potrntially used as the fluorophore and an DNBS group was combined as both a fluorescence quencher and a recognition unit .which can react with thiol and releases the hydroxypyrene which has strong fluorescence, The fluorogenic reaction is selectivel y initiated by the presence of thiols. The results indicate high application potential in analytical chemistry and diagnostics. Keywords : Thiols, Anchoring- Unanchoring, hydroxypyrene, DNBS, CTAB, Chemosensor. I. INTRODUCTION In last few decades there has been specific attention on the chemosensing of mercaptans, as they play a key role in extensive array of physiological processes and biological functions 1-5 ,biothiols such as cysteine ,homocysteine, and glutathione are a component of many amino acids and peptides, Preservation of appropriate concentrations of these low molecular-weight thiols is necessary for numerous cellular functions, conversely, the abnormal levels of thiols can give rise to various health complications. For instance, the inadequacy of Cys contribute to slowed growth in children, hair depigmentation, edema, lethargy, liver damage, loss of muscle and fat, skin lesions, and weakness,an elevated level of Hcy in human plasma is a risk factor for Alzheimer’s, cardiovascular diseases, neural tube defect, and coronary heart disease 6-14 . Therefore, a rapid, sensitive, and selective detection of thiols in biological samples is of significance. Typical instrumental detection methods for biothiols include liquid chromatography, capillary electrophoresis, voltammetry and flow injection 15-20 . However, those techniques require relatively complex sample preparation protocols and sophisticated instrumentation. Among various methods for detection of biothiols, the fluorescence method 21-41 based on fluorescence probe is expedient due to its desirable features such as high sensitivity, simplicity, and potential for in vivo imaging . Noteworthy efforts have been devoted to construction of fluorescent probes for biothiols . Many fluorescent probes, involved in different detecting mechanisms, have been developed for thiols detecting, such as Michael addition reaction 42 , cyclization reaction based on aminothiols and aldehyde , cleavage reaction induced by thiols ,and ligand displacement of metal complexes by thiols. 21-41 The thiol mediated nucleophilic displacement strategy 43 provides a superior performance of chemosensors for recognition of thiols. However, it is