IJSRST173820 | Received : 01 Nov 2017 | Accepted : 13 Nov 2017 | November-December-2017 [(3)8: 128-133] © 2017 IJSRST | Volume 3 | Issue 8 | Print ISSN: 2395-6011 | Online ISSN: 2395-602X Themed Section: Science and Technology 128 Mitrochondrial Cytochrome Oxidize I (mt COI) Diagnostic Technique for the Quick Identification of Biotypes Whitefly, Bemisia Tabaci (Gennadius) on different Crops Swati Tomar 1 , Kamlesh Malik 2 , Seema Sharma 1 1 Zoology Department, Meerut College, Meerut, Uttar Pradesh, India 2 Plant Protection, Central Potato Research Institute Campus, Modipuram, Meerut, Uttar Pradesh, India ABSTRACT Bemisia tabaci (Genn.) is one of the important pest and affects a board range of agricultural crops in the world having many biotypes. There is evidence that B. tabaci should be considered a cryptic complex species of 11 well- defined groups containing at least 24 morphologically indistinguishable species. Its populations differ biologically with respect to insecticide resistance, virus transmission and host range. Therefore, understanding genetic variation among populations is important for management. In present study, the mtCO I sequence of B. tabaci was analyzed from population sample collected from different host plants from different area, Uttar Pradesh. Results obtained showed that amplification of mtCO I gene fragment using the primers (C1-J-2195 and L2-N-3014) produced B. tabaci specific ~800bp band in all the samples of whitefly. Further Sequencing, homology search by Blast and comparison against the consensus sequences of Dinsdale or Ellango, revealed that these samples belong to group Asia II. It is highly probable that these all biotypes are the same entity, but have been assigned different names either as a consequence of the use of different methods of identification or a failure to broadly consider the available data in sources such as Genebank. Keywords : Amplification, Biotypes, COI, Bemisia tabaci I. INTRODUCTION Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a phloem-feeding insect that lives predominantly on herbaceous species. It is a pest of ornamental, vegetable, grain legume, and cotton production, causing damage directly through feeding (Oliveira et al., 2001) and indirectly through the transmission of plant pathogenic viruses, primarily begomoviruses (Jones, 2003). It has a global distribution (Boykin et al., 2007; Dinsdale et al., 2010). Bemisia tabaci complex is a cryptic species complex, composed of >35 morphologically indistinguishable species (Calvert et al., 2001), varies considerably in its ability to transmit geminiviruses (Bedford et al., 1994), rate of development (Wang & Tsai, 1996) and ability to utilize different host plants (Brown & Bird, 1995), but genetically distinct groups (Boykin et al., 2007 and Dinsdale et al., 2010), molecular (Xu et al., 2010) and biological data 5 supports their status as different species (De Barro et al., 2011). B. tabaci has a high intra specific biological and genetic variability (De Barro et al., 2000). Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex of about 41 biotypes (De Barro et al., 2011) out of which 5 (H, P, K, G, B) have been reported from India. It is vector of Apical Leaf Curl Virus (ALCV) in agricultural ecosystems. Among the biotypes, B biotype is most significant having the highest transmission efficiency for ALCV diseases. Biotype B, because of its global pest status, has been the focus of considerable research over the past many years. Previous studies of Genetics structure of B .tabaci in India were limited to samples collected from different regions only (Perring et al., 2001, Banks et al., 2001; Rekha et al., 2005, Lisha et al., 2003, Malik and Singh., 2007 Reddy et al., 2012, Singh et al., 2012 and Swati tomar et al., 2014). Molecular markers have been used to study the genetic polymorphism of B. tabaci and mtCOI gene is the most widely applied DNA region for determine the genetic structure of B. tabaci ( Ellango, et al., 2015; Dinsdale, 2010; De Barro et al., 2011;