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ANALYSIS OF DRUG CONTENTS OF CEFOTAXIME FORMULATIONS
AVAILABLE IN THE LOCAL MARKET AND THE
EFFECT OF STORAGE CONDITIONS
HADIER A. THAMER
1
, NIZAR S. HADDAD
2
& ABDULLAH M. JAWAD
3
1
Bsc (Pharm), Msc Student (Pharmacology), Department of Pharmacy, Al-Sader Teaching Hospital, Basrah, Iraq
2
MBCHB, CAMB, Assistant Professor in Chemical Pathology, College of Medicine, University of Basrah, Iraq
3
MBCHB Ph. D, Professor of Pharmacology, College of Medicine, University of Basrah, Iraq
ABSTRACT
Background: Low quality antimicrobial drugs represent a world-wide problem. Beta-lactams are the main types
of antimicrobials found to be substandard or counterfeit. Environmental conditions can play an important role in
maintaining drug safety and quality .
Aim: To investigate whether the pharmaceutical formulations of cefotaxime, available in the local drug market,
contain appropriate amounts of the active ingredients, and how their contents are changed after being exposed to different
storage conditions.
Methods: Five brand names of cefotaxime vials available in authorized pharmacies in the local drug market, are
selected. Drug contents were measured by HPLC (Agilent, UK) using C18 column. Other tests performed include mass
uniformity (weight) test, pH of reconstituted solution, identification of impurities, and color of solid dosage forms.
Samples were re-analyzed after being exposed to different temperature and humidity levels for 30 days .
Results: No substandard and/or counterfeit drugs were found among the tested samples of cefotaxime.
The amount of drugs of all tested samples was more than 90% of the labeled one. Tested samples of the drug were stable
when stored at a temperature of 35 C˚ for 30 days. When they were stored at temperature of 50 C˚ for 30 days, part of the
active ingredients was lost which reached up to 7.4%, with variation between the 5 brands tested. This caused the active
ingredients in two brands to fall below the acceptable 90% of the labeled amount. The effect of humidity on the stability of
collected samples of different packaged brands of cefotaxime was insignificant .
Conclusion: The finding that none of the 5 brands of cefotaxime are of low quality is reassuring and can give
the treating physician the choice among different brands of these drugs according to price and availability. Care should be
taken in storing or transporting these drugs at high (50 C˚) temperature.
KEYWORDS: Low Quality Antimicrobials, Counterfeit Medicines, Beta-Lactam Antibiotics, HPLC & Cefotaxime
Received: Jan 22, 2018; Accepted: Feb 12, 2018; Published: Mar 03, 2018; Paper Id.: IJMPSAPR20183
INTRODUCTION
Low quality medicines such as substandard or counterfeit medicines become a global problem with
increasing rate in the last decade [1]. According to both the United States Food and Drug Administration (US-FDA)
and the World Health Organization (WHO), up to 10% of drugs worldwide are low quality medicines [2]. In
developing countries, counterfeit medicines account for 10 to 30% of all medicines used, with rates higher than
Original Article
International Journal of Medicine
and Pharmaceutical Science (IJMPS)
ISSN (P): 2250-0049; ISSN (E): 2321-0095
Vol. 8, Issue 2, Feb 2018, 11-18
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