Copyright © Lauree Ann Duprey-Reed Lauree Ann Duprey-Reed 1 , Mahendra Kumar Trivedi 1 , Alice Branton 1 , Dahryn Trivedi 1 , Gopal Nayak 1 , Mayank Gangwar 2 and Snehasis Jana 2 * 1 Trivedi Global Inc, USA 2 Trivedi Science Research Laboratory Pvt Ltd, India *Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt Ltd, Bhopal, India, Email: Submission: June 26, 2018; Published: August 01, 2018 A Comparative Analysis of Vitamin D 3 and Biofield Energy Treated Vitamin D 3 in Bone Health Using MG-63 Cell Line Research Article Orthoplastic Surgery & Orthopedic Care International Journal C CRIMSON PUBLISHERS Wings to the Research 1/7 Copyright © All rights are reserved by Lauree Ann Duprey-Reed. Volume 1 - Issue - 5 Introduction Our good health depends on various factors such as adequate input of essential vitamins and minerals. One of the extreme import- ant vitamin, is vitamin D not only to strengthen bone and teeth and bones, but also provide protection against serious diseases. Some primarily function of vitamin D included the calcium absorption, which helps to build bones strong and healthy [1]. Besides, it also blocks parathyroid hormone release in the body, which protects the body from reabsorbing the bone tissue and lead to bones thin and brittle. Vitamin D receptors (VDRs) are present in everybody or gans and significantly regulate the cell-to-cell communication, cell differentiation, hormonal balance, cell cycling and proliferation [2]. The synthesis of vitamin D is complex process and the energy of ultraviolet B rays in sunlight would convert the cholesterol pres- ent in skin into vitamin D 3 (inactive form), which travels through blood to liver to form 25-hydroxy-vitamin D. Further, in kidney it produces 1,25-dihydroxy vitamin D, also known as calcitriol (bio- ISSN 2578-0069 Abstract Poor bone health is related with various bone disease that predisposes to fractures, while sufficient supplementation with calcium and vitamin D suggested prevention of bone diseases. The present study aimed to find the effect of Consciousness Energy Healing based vitamin D 3 and DMEM medium on in vitro bone health parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity, collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D 3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability range 76% to 139% with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by 171.8% and 189.7% at 10µg/mL in the UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In addition, 100% and 33.3% increased ALP at 0.1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. The level of collagen was significantly increased at 10µg/mL by 145.8% in the UT-DMEM+BT-TI group, while 65.8% and 145.8% at 1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 86.3% and 136.1% at 1 and 10µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 150%, 21%, and 73.2% at 0.1, 10, and 100µg/mL, respectively in the UT- DMEM+BT-TI group, while 258.3% and 126.8% at 0.1 and 100µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 33.3%, 32.7%, and 187.4% at 0.1, 10, and 100µg/ mL, respectively as compared with the untreated group. Biofield Energy Treatment might be vital in promotion and maintenance of strong and healthy bones and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer, and many autoimmune disorders. Keywords: Biofield energy; Bone strength; Osteosarcoma cells; Vitamin D; Bone mineralization Abbreviations: CAM: Complementary and Alternative Medicine; NCCAM: National Center for Complementary and Alternative Medicine; MG-63: Human Bone Osteosarcoma Cells; ALP: Alkaline Phosphatase; DMEM: Dulbecco’s Modified Eagle’s Medium; FBS: Fetal Bovine Serum; FBS: Fetal Bovine Serum; EDTA: Ethylenediaminetetraacetic Acid; UT: Untreated, BT: Biofield Energy Treated, TI: Test Item