IJSART - Volume 3 Issue 11 – NOVEMBER 2017 ISSN [ONLINE]: 2395-1052 Page | 1267 www.ijsart.com Isolation of Endophytic Microorganisms As A Source of Novel secondary Metabolite Producers Against Tuberculosis R.S.Mane 1 , M.B.Shinde 2 , P.R.Wagh 3 , H.M.Malkar 4 1 Sadashivrao Mandlik Mahavidyalaya Murgud (M.H) 2,3,4 S.A.C.S College, Kopargaon (M.H) Abstract- In the present research study, isolation of endophytic microorganisms were carried out from Fresh body parts of Azadirachta indica and Parthenium hysterophorus plants collected from botanical garden of SMM murgud (M.H).Total 10 endophytic microorganisms were isolated and named them as R1, R2upto R10. Among thisIsolates 6 was Bacterial species and 4 was fungal species. From this 10 isolates, R2, R3, R5 and R10 showed highest antimycobacterial, antifungal and antibacterial activity which was again selected for further study. From present research study it can be concluded that, endophytic microorganisms are able to produce secondary metabolites and that can be used indifferent clinical applications. Keywords- Endophytic microorganisms, Antifungal and Antibacterial activity, Secondary metabolites. I. INTRODUCTION The need for discovery of new and useful drugs to provide effective treatment and relief in all aspects of humankind is increasing due to arise of many drug resistance bacteria, the appearance of life-threading viruses, fungal infection, environmental degradation and loss of biodiversity (Strobel & Daisy, 2003).An endophyte is an organism which lives inside a plant. This is contrasted to epiphyte, which refer to the organism on the outside of the plant.while Wilson (1995) considered endophytes as any fungus or bacterium found inside plant tissues. Endophyte that occure intercellularly within the leaves,stems and reproductive organs of grasses have impressive effects on the physiology, ecology and reproductive biology of its host plant.They often protect their host plants against a wide range of insect and mammalian herbivores, through the production of toxic alkaloids.Endophytes are the chemical synthesizers inside plants. (Owen & Hundley,2004). Many of them are capable of synthesizing bioactive compounds that can be used by plants for defense against pathogens and some of these compounds have been proved useful for novel drug discovery.According to Demain (1981),antibiotics compound is low-molecular- weight organic natural products made by microorganisms that are active at low concentration against other microorganisms, are the most bioactive natural products isolated from endophytes.In the present research, isolation and antimicrobial activity of endophyticbacteria from different body parts of Azadirachta indica is performed. II. MATERIALS AND METHODS Collection of samples Fresh body parts of Azadirachta indica and Parthenium hysterophorus was collected from the botanical garden of SMM murgud (M.H). Samples were immediately brought to the laboratory and used within 24 hrs for the isolation of endophytic microorganisms. Surface sterilization of samples The leaves and roots were thoroughly washed in running tap water and immersed in 70% ethanol for 10 seconds, followed by 4% sodium hypochlorite for 1 minute and then rinsed in sterile distilled water for 1 minute; the excess moisture was blotted with a sterile filter paper. sterilized Samples were excised with sterilized scalpel and forceps under aseptic condition and dried for further use. Sterility check To confirm that sterility of sterilized Samples 0.1 ml of the sterile distilled water that was used in the final rinse of surface sterilization process were spreaded on sterilized NA media plates and incubated at 37 0 C for 72 hrs. Preparation and sterlization of media Nutrient agar media were prepared by the addition of nutrient broth and agar agar powder in distilled water.