Int. J. Adv. Res. Biol. Sci. (2018). 5(8): 186-195 186 International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com DOI: 10.22192/ijarbs Coden: IJARQG(USA) Volume 5, Issue 8 - 2018 Research Article Prevalence and antibiotic resistance pattern of Pseudomonas and Acinetobacter spp. isolated from blood samples of the intensive care unit (ICU) patients Md. Jakir Hossain 1 , Md. Gahangir Alam 2 , Shashwata Biswas 3 , Sirajo Ismail Isah 4 , Mohammad Shahriar 5 and Sohidul Islam 1* 1 Department of Biochemistry and Microbiology, North South University, Dhaka 1229, Bangladesh. 2 Department of Biochemistry & Molecular Biology, Gono Bishwabidalay, Savar, Dhaka 1344. Bangladesh. 3 Center for AIDS Research, Kumamoto University, Kumamoto 860-0811, Japan. 4 Department of Pharmacy, Daffodil International University, Dhaka 1207, Bangladesh. 5 Department of Pharmacy, University of Asia Pacific, Dhaka 1215, Bangladesh. * Corresponding author: Dr. Sohidul Islam. E-mail: mjh_nsu@yahoo.com Abstract Pseudomonas and Acinetobacter spp. are opportunistic pathogens and primarily cause opportunistic nosocomial infections and affects severely immunocompromised patients. In this study, we observed the prevalence and resistance pattern of Pseudomonas and Acinetobacter spp. from the blood of intensive care unit (ICU) patients. Isolates of both the organisms were collected during one year period from January to December 2017. MacConkey and Blood agar were used to isolate these bacteria. Different biochemical tests (KIA, MIU, Citrate, and Oxidase tests) were carried out to identify the organisms and polymerase chain reaction (PCR) was performed to correlate and confirm the previous results. After identifying the organisms statistical analysis was done to observe the prevalence based on age and sex. Antibiogram was studied against amikacin (AK), ceftazidime (CAZ), ciprofloxacin (CIP), gentamicin (CN), imipenem (IMP), meropenem (MEM), doripenem (DOR), and netilmicin (NET). A total of 198 Pseudomonas spp. and 57 Acinetobacter spp. were isolated from blood of 312 patients. Both the bacteria were most prevalent among male patients of different ages. In antibiotic resistance experiment, Pseudomonas spp. was found to have reduced susceptibility (RS) against most of the antibiotics used especially ceftazidime (CAZ) and netilmicin (NET) and 6.76% isolates were found to be resistant against gentamicin (CN). In case of Acinetobacter spp., reduced susceptibility (RS) was observed against netilmicin (NET) and 52.63% of isolates were found to have resistance against gentamicin (CN).The presence of Pseudomonas, and Acinetobacter spp. were in notable range especially among male patients and both the bacteria showed reduced susceptibility (RS) against most of the antibiotics used and total resistance against gentamicin (CN). Keywords: Pseudomonas spp., Acinetobacter spp., and antibiotic resistance. DOI: http://dx.doi.org/10.22192/ijarbs.2018.05.08.017