CH16119 | Received: 13 Sept. 2016 | Accepted: 24 Sept. 2016 | September-October 2016 [(1)1: 35-38 ] International Journal of Scientific Research in Chemistry (IJSRCH) | ISSN : 2456-8457 © 2016 IJSRCH | Volume 1 | Issue 1 35 Synthesis and Characterizations of N 2 ,N 4 -bis(5-nitro-1,3-benzothiazol- 2-yl)-N 6 -aryl-1,3,5-triazine-2,4,6-triamine,as Biological Agents J. S. Makwana, Dr. B.B.Baldaniya Chemistry Department, M G Science Institute, Navarangpura, Ahmedabad, Gujarat, India ABSTRACT Some novel N 2 , N 4 -bis (5-nitro-1,3-benzothiazol-2-yl) - N 6 - aryl-1, 3, 5-triazine-2, 4, 6-triamine 1a-1l have been synthesized and characterized by elemental analyses IR and NMR spectra. The products tested for their antibacterial activity against gram (+)ve and gram (-)ve bacteria. Introduction of –OH, -NO 2 , -Cl and –Br groups to the heterocyclic frame work enhanced antibacterial activities. Keywords: 1, 3, 5-triazine-2, 4, 6-triamines; Antibacterial activity. I. INTRODUCTION Antibacterial and Antiviral diseases are very common in all over the world. The s-triazine based chalcones and their derivatives have been studied extensively because of their wide range of biological activity. The s-triazine 1- 6 have been linked with a wide range of therapeutic activities 7-12 such as Antibacterial, Fungicidal, Anticancer, Antitubercular. Among all heterocycles, nitrogen based heterocycles have specific and unique identity in the world. Pyrimidine, oxadiazole, coumarin, pyrimidine, s-triazine are some of the examples. The research work described here is humble effort to synthesis the nitrogen based novel heterocycles. And study of their pharmaceutical importance in medicinal chemistry. The study of pyrazoline derivatives has been a developing field within the heterocyclic chemistrybroad spectrum of biological activity 13-20 . Pyrazoline derivatives have been found to be bactericidal 13,14 , fungicidal 15,16 , and insecticidal agents 17,18 . Asurveyof more recent literature reveals that some pyrazoline derivatives possess cerebroprotective properties 19 and antidepressant activity 20,21 . It is our project to produce new bioactive molecules. Currently used antibacterial agents are not effective due to the resistance developed by the bacterial. And therefore, it is an ongoing effort to synthesize new antibacterial agents. In view of these observations we have synthesized s- triazine, 4a-m (scheme-1, Table -1) by the condensation of triazine with different aromatic amines. 6-chloro- N,N'-bis (5-nitro-1,3-benzothiazol-2-yl)-1,3,5-triazine- 2,4-diamineafforded the title compounds 1a-1l respectively (scheme -1) the series of compounds were characterization by IR and NMR analysis. II. METHODS AND MATERIAL Biological Activity Antibacterial Activity: Antibacterial activity was carried out by broth dilution method. Antibacterial activity was carried out by broth dilution method 22 . Concentrations of 1000, 200, 100, 50, µg/ml respectively (Table 2) of compound 1a-1l. Antifungal Activity: Same compounds were tested for antifungal activity against C. Albicans A.Niger and A. Clavatus at concentrations of 1000, 500, 200, and 100 and 50 µg/ml respectively (Table 2) of compound 1a-1l. The results are recorded in the form of primary and secondary screening. Each synthesized drug was diluted to obtain 1000 µg/mL concentration, as a stock solution.