Journal of Clinical Microbiology and Biochemical Technology CC By 001 Citation: Muthu R, Rajan MK, Rajeshwari S (2018) Modulation of Immune Response in Edible Fish against Aeromonas hydrophila. J Clin Microbiol Biochem Technol 4(1): 001-005. DOI: http://dx.doi.org/10.17352/jcmbt.000030 Clinical Group http://dx.doi.org/10.17352/jcmbt.000030 DOI Abstract The present study was to obtain a basic knowledge of the hematology of Catla catla Post challenged with Aeromonas hydrophila. Fish were fed diets representing different supplementation levels of Aegle marmelos fruit extract. The various concentration of fruit extract were 10mg, 20mg and 30mg per 100g for each diet of fish. Supplementation of experimental feed after seven days collected from serum in treated fish to haematological parameters and biochemical parameters were analysed between control and experiment. The results of challenge test suggest that the fishes fed with 30mg Aegle marmelos diet had better immunostimulatory activity compared to the control group. Thus, our finding confirms our concentration that Aegle marmelos is a growth promoter and immunostimulant. Research Article Modulation of Immune Response in Edible Fish against Aeromonas hydrophila R Muthu*, Rajan MK and S Rajeshwari Department of Zoology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, India Received: 09 January, 2018 Accepted: 17 January, 2018 Published: 18 January, 2018 *Corresponding author: R Muthu, Department of Zoology, Ayya Nadar Janaki Ammal College (Autono- mous), Sivakasi, India, E-mail: Keywords: Catla catla; Aegle marmelos; RBC; WBC; Protein and lipid https://www.peertechz.com Introduction Aquaculture is one of the important sectors contributing significantly in the Indian economy. Fish culturists are encouraged towards intensification of culture system to increase production and profit. In such practice of fish and shrimp farming, disease becomes major threats. Disease is one of the most important constraints of fish production both in culture system, as well as in wild condition [1]. For the last twenty years, the problem of microbial diseases has emerged as a major constraint to aquaculture industry. Increased disease occurrences have resulted due to the transfer of pathogenic organisms among cultured species of fish and shrimp, between different countries without proper quarantine measures. Due to this, the fish industry in India as well as other Southeast Asian countries has suffered significant economic losses [2]. Fish production is decreased due to the occurrence of disease caused by different pathogens in aquaculture. Aquaculture has been a tradition in several parts of Asia and according to FAO statistics, over 80% 0f fish produced by aquaculture come from Asia, where the production was 31.07 million metric tons valued at $ 38.855 billion [4]. Aeromonas hydrophila is a gram negative motile bacterium. The ulcerative disease is mostly caused by gram negative bacterium. Aeromonas hydrophila is pathogenic not only to fishes but also to amphibian, reptiles and mammals including man [5]. Aeromonas sp. is a ubiquitous inhabitant of aquatic ecosystems such as, freshwater, coastal water and sewage. These bacteria are usually microbiota as well as primary or secondary pathogens of fish and amphibians. Some motile species of Aeromonas, such as, Aeromonas caviae, A.hydrophila and A.veronii are opportunistic pathogens of humans. Among the species belonging to Aeromonas genus, one of the most important is A. salmonicida, a fish pathogen which causes a common disease among salmonids, named furunculosis or ulcerative furunculosis [6]. The Indian major carps, Catla catla, Labeo rohita and Cirrhinus mrigala are the most important commercial fishes in India with a maximum market demand and acceptability as food by the consumers due to their taste and flesh. They contribute about 67% of total freshwater fish production [7]. Catla catla and Labeo rohita contribute a major portion to the freshwater fish production in South India. The Indian major carp Catla catla mainly inhabits in rivers. It can also be easily cultured in ponds and lakes Catla is non- predatory and its feeding is restricted of the Catla enables its safe introduction into fish community including Rohu, Mrigal, etc. despite its reputations as a vegetarian species [8]. Aegle marmelos, a plant indigenous to India has been used by the inhabitants of the Indian subcontinent for over 5000 years. The leaves, bark, roots, fruits and seeds are used extensively in the Indian traditional system of medicine the Ayurveda and in various folk medicine to treat myriad ailments. Bael fruits are of dietary use and the fruit pulp is used to prepare delicacies like murabba, puddings and juice. Bael fruits are also used in the treatment of chronic diarrhea, dysentery, and peptic ulcers, as a laxative and to recuperate from respiratory affections in various folk medicines [9]. Hence, the present study has been carried out the Haematological studies on disease induced Indian major carp; Catla catla (L) fed with Aegle marmelos formulated diet.