~ 27 ~ International Journal of Unani and Integrative Medicine 2018; 2(3): 27-31 E-ISSN: 2616-4558 P-ISSN: 2616-454X IJUIM 2018; 2(3): 27-31 Received: 07-05-2018 Accepted: 09-06-2018 Md. Shariful Islam Department of Pharmacy, Southeast University, Dhaka, Bangladesh Md. Atikur Rahman Ope Department of Pharmacy, Southeast University, Dhaka, Bangladesh Mohshin Kabir Department of Pharmacy, Southeast University, Dhaka, Bangladesh Sabiha Ferdowsy Koly Department of Pharmacy, Southeast University, Dhaka, Bangladesh Md. Morad Biswas Department of Pharmacy, Southeast University, Dhaka, Bangladesh Shaikat Hossain Department of Pharmacy, Southeast University, Dhaka, Bangladesh Md. Sharif Ahammed Department of Pharmacy, Southeast University, Dhaka, Bangladesh Sadia Afrin Department of Pharmacy, Southeast University, Dhaka, Bangladesh Correspondence Md. Shariful Islam Department of Pharmacy, Southeast University, Dhaka, Bangladesh Estimation of anti-inflammatory, analgesic, thrombolytic activities of Sonneratia caseolaris linn. (Family: sonneratiaceae) Md. Shariful Islam, Md. Atikur Rahman Ope, Mohshin Kabir, Sabiha Ferdowsy Koly, Md. Morad Biswas, Shaikat Hossain, Md. Sharif Ahammed and Sadia Afrin Abstract In this study, Sonneratia caseolaris was evaluated for its anti-inflammatory, analgesic and thrombolytic activities using different systems assay. The anti-inflammatory activity, the % of inhibition of paw edema of crude methanol extract showed the highest activity in leaf (58.24% inhibition) at 100 μg/ml, in bark (40.80% inhibition) at 200 μg/ml. The analgesic activity, the effect of each extractive at two different concentration (100 & 200 μg/ml) on the response of mice was measured. The most potent activity was also found in crude methanolic fraction (CME) showed in leaf highest % of inhibition (25.93 at 100 μg/ml) and in bark highest % of inhibition (33.33 at 100 μg/ml) after standard (Diclofenac-Na). Hence, it is worthwhile to isolate and elucidate the bioactive principles that are responsible for the better activity that is underway. Keywords: Anti-inflammatory activity, paw edema, analgesic activity, Sonneratia caseolaris 1. Introduction The variety of medicinal plants is increasing around the world includes more than a thousand species [1] . In Bangladesh more than 500 of these medicinal plants have been reported right now. Medicinal plants have long been extensively used in preparation of ayurvedic, unani, homeopathic medicines in our country and the proper scientific evaluation of the species limits their uses in therapeutic applications. S. caseolaris is a mangrove [1] species belonging to family Sonneratiaceae [2] . S. caseolaris is a widespread and can be found in Bangladesh, China, Indonesia, India, Malaysia, Philippines, Myanmar, Singapore, Sri Lanka, Thailand, Northeast Australia, Cambodia, Maldives, Solomon Islands and New Caledonia. S. caseolaris is a medium is medium-size plant (2-20m hight), evergreen tree with elliptic- oblong leaves (5-9.5cm long) [3-4] . Flowers solitary at the end of braches about 5cm across and fruits are a fleshy berry supported by persistent calyx, depressed globose (5-8cm across). S. caseolaris have 24 compounds such as ninetriterpenoids, eight steroids, three flavonoids and four benzene carboxylic derivatives have been isolated from stems and twigs of medicinal mangrove plant S. caseolaris [5] . This plant contains phenolic compound like gallic acid and flavonoids e.g. luteolin and luteolin-7-O-glucoside [6] . S. caseolaris to be used in traditional medicine systems in several countries, it is used for sprains, swelling helminthiasis, poultices, coughs, hematuria, small pox, astringent, antiseptic, arresting hemorrhage, piles, and also used as remedy to stop blood bleeding [7] . S. caseolaris possessed intestinal α-glucosidase inhibitory property [9] and it has also been reported to be toxic against mosquito larvae [7] . The present study was started to identified the pharmacological parameter of extracts from mature leaves and barks of S. caseolaris because that it may contains substances with potential therapeutic uses and these could serve a basis of precursors for synthesis of useful drugs. 2. Materials and Methods 2.1.1 Plant material: The leaves of S. caseolaris were collected from Barisal districk, Bangladesh, in April 2014, and identified by an expert taxonomist. A voucher specimen was submitted to the national herbarium, Mirpur, Dhaka, Bangladesh. 2.1.2 Preparation of the extract: Powdered plant materials (leaves) having a weight of about 340gm were taken in an amber colored reagent bottle and soaked in 1.5 liter of