IJIRST –International Journal for Innovative Research in Science & Technology| Volume 5 | Issue 1 | June 2018 ISSN (online): 2349-6010 All rights reserved by www.ijirst.org 22 Studies on Biosorption of Titan Yellow Dye with Hypnea Musciformis Powder and Optimization Through Central Composite Design Dr. Ch. A. I. Raju K. Sunil Assistant Professor Research Scholar Department of Chemical Engineering Department of Chemical Engineering Andhra University, Visakhapatnam – 530 003, AP, India Andhra University, Visakhapatnam – 530 003, AP, India Abstract The major three resources like Air, Water and Food have been polluted and are seeking a special attention for their originality which has to be reestablished. The present research was investigated using Hypnea musciformis powder as a potential biosorbent for the removal of Titan Yellow. The operating parameters involved are agitation time, biosorbent size, and pH of the solution, initial concentration of solution, dosage of biosorbent and temperature of the solution. The optimization was also incorporated u n of INDIGO CARAMINE is 20 mg/L were compared using one factor at a time with CCD. The kinetics and isotherm studies are also studied along with thermodynamic study. Keywords: Hypnea musciformis; Titan Yellow; biosorbent; CCD _______________________________________________________________________________________________________ I. INTRODUCTION Water – a priceless gift from the nature to the mankind. It is irreplaceable. The role of water in human life is noteworthy without which tasks such as running water for household activities, to rear cattle and farming, or for the industrial usage remain dormant [1].There may be various reasons causing the degradation in quality of water day by day which include weathering, dissolution, precipitation, ion exchange, various biological processes, Sewage leakages ,high population density, oil spillage, Industrial waste dumps, pollution of ground water through drilling activities, flooding during rainy season which carries waste deposits into our waters, radioisotopes ,Heavy metal, Combustion, Toxic waste disposal at sea ,Deforestation , Mining Littering ,Pesticides ,herbicides and fertilizers ,Failing septic system ,House hold chemicals ,Animal wastes [2]. In the recent years, population increase has been sharp and both the industrial and domestic needs of people increased tremendously [3].Out of the several uses of water, drinking purpose holds a major role and ground water stands as a major source for drinking needs in most parts of India accounting for about 88% of safe drinking water in rural India For drinking and even 45% irrigation water is supplied from groundwater [4]. There are various techniques in use to treat water that has been polluted such as screening, filtration and centrifugal separation, Sedimentation, gravity separation, coagulation, flotation, Aerobic, Anaerobic, distillation, crystallization, evaporation, solvent extraction, reverse osmosis, ultra filtration, electro dialysis etc [5]. Industrial discharge has been the major chunk of wastewater contributors this has been one of the main causes of irreversible ecosystem degradation[6].These facts remaining so, water consumption rates are increasing from 313 liters per capita day for the affluent to a mere 16 liters per capita day for the slum dweller [7]. The data obtained on monitoring quality of water exposed the fact that quality of water at most of the monitoring points is poor [8]. On evaluating the water quality for irrigation suggest that the majority of the groundwater samples are not good for irrigation in post monsoon this needs to addressed since agriculture contributes 46% to the gross national product [9].All these demand a sustainable utilization of water and its resources both effectively and efficiently without resulting into scarcity and degradation in the existing quality of water. II. EXPERIMENTAL PROCEDURE Preparation of the Biosorbent Hypnea musciformis algae were collected from Jodugullapalem beach in Visakhapatnam and were washed with water to remove dust and soluble impurities and dried in sun light till the algae became crispy and colorless. By passing it through a set of sieves ranging from 300 to 75 mesh sizes the dried algae were finely powdered and sized. The powder of 53, 75, 105, 125 and 152 micron meters were separated and stored in dry bottles with double cap and used as biosorbent. Preparation of 1000 Mg/L of Indigo Caramine Dye Stock Solution To prepare 1000 ppm of indigo caramine dye stock solution 1.0 g of 99 % Indigo Caramine dye powder was dissolved in 1.0 L