Green Synthesis of Silver Nanoparticles Using Phyto-Constituents of Ficus Auriculata Lour. Leaf Extract: Mechanistic Approach John J 1,2* , Aravindakumar CT 2 and Tomas S 3 1 Department of Biotechnology, CMS College, Kottayam, India 2 Interuniversity Instrumentation Centre, India 3 International and Interuniversity Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala, India * Corresponding author: John J, Department of Biotechnology, CMS College, Kottayam, Kerala, India- 686001, Tel: +91-9446664201, E-mail: jinujohn21@gmail.com Citation: John J, Aravindakumar CT, Tomas S (2018) Green Synthesis of Silver Nanoparticles Using Phyto- Constituents of Ficus Auriculata Lour. Leaf Extract: Mechanistic Approach. SAJ Biotechnol 4: 103 Green synthesis of silver nanoparticles from silver nitrate using Ficus auriculata leaf decoction as a reducing agent has been elucidated with possible mechanism of reaction. Preliminary phytochemical screening of the extract revealed the presence phenolics, carbohydrates, favonoids and saponins. HPTLC fngerprinting and further UPLC-Q-TOF mass spectroscopy analysis confrmed the presence of the favonoid glycoside, rutin in the extract. Te extract showed good antioxidant property in DPPH model. SEM and TEM analysis showed that the nanoparticles formed were of diferent morphologies with an average size of 19-21 nm. FT-IR spectral analysis showed the role of hydroxyl groups in the phytoconstituents as reducing agent in synthesis. Te synthesized silver nanoparticles showed good antimicrobial potential when analysed against Photobacterium leiognathi, a very sensitive and accurate biosensor, by luminometry. Synthesized silver nanoparticles showed good morphological, dimensional and pharmacological properties. . Abstract Keywords: Antioxidant Property; Ficus auriculata; Green Synthesis; Silver Nanoparticles RESEARCH ARTICLE Volume 4 | Issue 1 ScholArena | www.scholarena.com SAJ Biotechnology ISSN: 2375-6713 Open Access Te antioxidant polyphenolics such as rutin presents in the F. auriculata leaf extract acted as reducing agent in the green synthesize of silver nanoparticle. Possible mechanism of action and bioactivity are also elucidated. Graphical Abstract Introduction Silver nanoparticles (AgNPs) exhibit improved properties such as plasmon resonance characteristics depending upon their size and morphologies [1]. Silver nanoparticles become the focus of intensive research owing to their wide range of applications in catalysis, optics, electronics, antimicrobials, cosmetics, biosensor fabrication and biomedical applications [2]. Tere are several reports on the antioxidant, cytotoxic properties of AgNPs and its potential applications as a drug carrier for the treatment of Article history: Received: 20 November 2017, Accepted: 27 December 2017, Published: 29 December 2017