IJSTE - International Journal of Science Technology & Engineering | Volume 5 | Issue 4 | October 2018 ISSN (online): 2349-784X All rights reserved by www.ijste.org 101 Antibacterial & Antioxidant Properties of Leave & Stembark Extract of Artocarpus Heterophyllus as the Component of Peel-Off Mask Ahmad Budiman Siregar Rumondang Bulan Department of Chemistry Department of Chemistry Graduate School-Department of Chemistry, Universitas Sumatera Utara Universitas Sumatera Utara. PUI Minyak Atsiri Eucaliptus Yuniarti Yusak Department of Chemistry Universitas Sumatera Utara Abstract Antibacterial and antioxidant activities have been conducted using methanol extract of leave and stem bark of Artocarpus for its application as peel-off mask. The raw material was macerated using methanol and the obtained filtrate was evaporated until the concentrated crude extract was obtained. The crude extract is tested using several tests, such a phytochemistry screening, antibacterial test to Propionibakterium acnes and Staphylococcus aureus with the concentration of extract of 50; 100; 150 and 200 ppm, and antioxidant test using DPPH method. The additional test was conducted for evaluating the characteristic of peel-off mask, such as homogeneity, pH, organoleptic and irritation test. The result of photochemistry screening showed those extracts were dominated with tannin and saponin. The antibacterial test to P. acnes showed the leave extract with concentration of 50, 100, 150 and 200 ppm showed the inhibition zone 9,6; 9,9; 10,4 and 11,3 mm. The other extract, the stem bark extract, provided the inhibition zone 8,4; 8,8; 9,5 and 10,5 mm at the similar concentration of extract. For Staphyliococcus aureus, the leaves extract showed the inhibition zone 7,5; 7,8; 8,2 and 8,8 mm, while the stem bark extract 6,2; 6,4; 7,7 dan 9,2 mm. Those result revealed that the leave extract is more powerful to inhibit P. acnes than stem bark extract. But the different result was obtained when discussed about the inhibition of S. aureus, the stem bark extract showed the higher activity than leave extract. The measurement of antioxidant of leave extract at concentration 50; 100; 150 and 200 ppm showed the following activity data 22,91; 31,90; 32,76 and 40,47%, then the stem bark extract has the following activity data 43,89; 64,66; 70,02; 70,44%. These data showed the antioxidant activity of stem bark extract is higher than stem bark extract. The clinical testing of peel-off mask formulation showed that the F1, F2 and F3 has a good homogeneity and the pH was 6,7; 6,7 and 5,8. The irritation test showed a negative reasult for all of formulation. Keywords: Antibacterial, Antioxidant, P. Acnes, S. Aureus, Jackfruit ________________________________________________________________________________________________________ I. INTRODUCTION The problem that being faced in the field of medicine today is the bacterial resistance against antibiotics in the developing and developed countries. Therefore, a lot of research has been conducted that focused in the production of antibiotics from the synthetic process and secondary metabolite from natural product to deal with the bacterial resistance [1]. Bacteria are microorganisms that cannot be seen with the naked eye, but can only be seen with a microscope. Among them are Staphylococcus aureus and Propionibacterium acnes bacteria. Staphylococcus aureus is a coccus-shaped bacterium with a diameter of about 1 μm and it arranged in irregular groups [2]. In human, Staphylococcus aureus is found in the nose, skin, throat, an d others. These bacteria can cause various infections, such as pneumonia, meningitis, empyema, endocarditis, zits, pyoderma, or impetigo [3] [4]. Propionibacterium acnes is a rod-positive gram-bacteria and it is a normal skin flora that plays a role in the formation of acne [5]. Propionibacterium acnes secretes hydrolytic enzymes which cause damage to the polysebaceous follicles and produce lipase, hyaluronidase, protease, lecithinase and neurimidase, which play an important role in the inflammatory process [6]. One kind of natural product that mostly used by people for treating any kind of health problem, including infection that caused by bacteria is leave and stem bark jackfruit (Artocarpus heterophyllus Lam.) The leave of jackfruit contains flavonoid, saponin and tannin that can act as antimicrobial and induce the production of new skin cell [7]. Also, the stem bark of jackfruit has some secondary metabolites, such as morin, sianomaklurin, flavonoid and tannin. Especially for the flavonoid that found in stem bark of jackfruit, there are some new compounds that has been discovered, such as morusin, artonin E, artokarpin, cycloartobiloxanton,