Multidisciplinary Advances in Veterinary Science Research Article MB Abubakar 1 *, RM Aliyu 1 and A Bello 2 1 Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto - Nigeria 2 Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto, Nigeria Received: October 26, 2016; Published: December 28, 2016 *Corresponding Author: MB Abubakar, Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto – Nigeria. Prevalence of Antifungal-Resistance Among Fungal Agents Isolated in Poultry-Feeds from Poultry Farms in Sokoto, Nigeria. Abstract The aim of this study was to determine the prevalence of fungal agents isolated from poultry feeds for antifungal-resistance. A total of 275 fungal isolates comprising of Aspergillus spp, (n = 135), Penicillium spp (n = 50), Rhizopus spp (n = 35), Fusarium spp (n = 20) and Mucor (n = 35) isolated from poultry feeds in Sokoto state, Nigeria were screened for resistance to Fluconazole, Nystatin and Voriconazole, using disk difusion method. The result shows that 12.7% of each fungal genera were susceptible to all the antifungal agents tested. Resistance to at least one or more antifungal agent were expressed by 85.2% of the Aspergillus spp., 80% of Penicillium spp. and 75% of Fusarium spp, with 51.9%, 70% and 50% of them showing multiple drug resistance to two or more antifungal agent tested. Antifungal resistance was found to be expressed by all (100%) of Mucor and Rhizopus spp respectively, with > 70% showing multiple drug resistance. The resitance was found to be more for fluconazole than Voriconazole. Regular mycological analysis of poultry feeds shall be imposed to ensure quality and safety of poultry feeds in the study area. Citation: MB Abubakar., et al. “Prevalence of Antifungal-Resistance Among Fungal agents Isolated in Poultry-Feeds from Poultry Farms in Sokoto, Nigeria”. Multidisciplinary Advances in Veterinary Science 1.1 (2016): 21-26. Volume 1 • Issue 1 • 2016 Page 21 to 26 Volume 1 Issue 1 December 2016 © All Copy rights are reserved by MB Abubakar., et al. Keywords: Antifungal-Resistance Fungal-agents; Multi-drug resistance; Moulds; Poultry-feeds Introduction The indiscriminate use of anti-fungal agents for the prevention of poultry diseases may contribute to the emergence of moulds and yeasts that are resistant to these agents (Reis., et al. 2012). Poultry feed quality is an important pre-requesite for achieving optimal pro- duction results and preservation of health condition of the birds (Abo-Shama., et al. 2015). Moulds and mycotoxin contamination of feed and feed ingredients occur worldwide, and because of the ubiquitous nature of these microorganisms they cannot be totally eliminated from feed ingredients (Trenholm., et al. 1988). Presence of mycoflora especially moulds in animal feed indicate a potential threat not only to feed quality but also to the well-being of animals that are being fed with it (Beuchat., et al. 1978). Although infection with pathogenic yeast such as Candida albicans and Cryptococcus neoformans are the most common invasive and opportunistic mycotic diseases (Denning., et al. 1998), filamentous fungi such as Aspergillus fumigatus are emerging as prominent agents