Journal of Pharmacy and Pharmacology 6 (2018) 395-402 doi: 10.17265/2328-2150/2018.04.010 Analysis of Carbamates Pesticides: Immunogical Technique by Local Development of Enzyme-linked Immuno-Sorbent Assay N. Bellemjid 1, 2 , A. Iddar 1 , A. Moussaif 1 , N. El Abbadi 1 and A. Mesfioui 2 1. National Centre for Nuclear Energy Sciences and Technologies, Rabat 10112, Morocco 2. Faculty of Sciences University IbnTofaïl, Kenitra 14000, Morocco Abstract: Carbamates insecticides are used increasingly in agriculture as a replacement for environmentally more persistent organochlorine insecticides for the control of insect pests. It was the most used pesticides in Loukkous-Morocco. Conventional methods employed to detect/analyze carbamates residues are time consuming and require sophisticated equipment only available in well-equipped laboratories. In addition, the conventional methods usually require a lot of complex pre-treatment of samples. Therefore, convenient and rapid pesticide detection system is urgently needed. In this order, we aimed to develop a rapid ELISA (enzyme-linked immuno-Sorbent Assay) for detection of some carbamates such as carbendazim [methyl 2-benzimidazole carbamate] and carbofuran [2,3-dihydro-2,2-dimethyl-7-benzofuranylmethyl carbamate]. To develop an immunoassay for carbendazim and carbofuran we have synthesized molecules with acid function (haptens with 4 and 5 carbons) that are coupled with BSA protein and injected to the rabbits, collected antibodies are used for the achievements of the immunoanalytical assay. Key words: Carbamates, polyclonal antibodies, ELISA. 1. Introduction Pesticides are defined as any substance or mixture of substances intended for preventing, destroying, repelling or mitigating any pest [1]. They are used to protect agriculture and horticultural corps against damage and also used as domestic insecticides [2], excessive use of pesticides affects human’s health, animals and environment. They have many different modes of action, but in general cause biochemical changes which interfere with normal cell functions. The carbamate family of pesticides is registered for use on several crops in South American and European Countries, and in the USA. It was the most used pesticides in Loukkous-Morocco [3]. Its use for pest control has increased progressively in recent years, together with the OPs (organophosphorus), as Corresponding author: A. Iddar , Ph.D., researcher, research fields: biochemistry and biotechnology. alternatives to OC (organochlorine) insecticides. Owing to their broad spectrum of biological activity, carbamates can be used as insecticides, miticides, fungicides, nematocides, and molluscicides [4]. Some are suspected carcinogens and mutagens [5]. Carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranylmethyl carbamate) (Fig. 1) is a pesticide widely and effectively used to control insects, but it is a potent cholinesterase inhibitor and thus exhibits a high toxicity to human beings and wildlife. Because of its widespread use carbofuran residues are potentialair, soil, water and food pollutants [6, 7]. Carbendazim (methyl- 1H-benzo- [d]- imidazol-2-yl-carbanate) (Fig. 2)is used as fungicide to control a broad spectrum of diseases on arable corps, fruits and vegetables, usable also in post-harvest food storage and as a seed pre-planting treatment. Toxic effects of carbendazim in humans and animals have been reviewed [8]. D DAVID PUBLISHING