International Scientific and Practical Conference “WORLD SCIENCE” ISSN 2413-1032 http://ws-conference.com/ № 6(22), Vol.5, June 2017 65 VETERINARY SCIENCE AND PHARMACY COMPARATIVE ANALYSIS OF QUALITY CONTROL OF FIBRINOGEN OBTAINED BY TWO DIFFERENT METHODS Master degree 2 course Aldabergenov D., PhD Turgumbayeva A., Doctor of Pharmacy science Ustenova G. Kazakhstan, Almaty, S.D.Asfendiyarov Kazakh National Medical University Abstract. Blood coagulation is very complex process that involves many proteins and coagulation factors. Since many people are suffering from difficult disorders, traumas and injuries, their coagulation process faces problems. The main protein that plays a crucial role in this process and has a hemostatic activity is known as fibrinogen. There are numerous methods to isolate fibrinogen and design better application to patients but quality control has to be carried out in order to verify which method can preserve its pharmacological activity. The study conducted in this article was done to compare fibrinogen properties obtained by two different methods. Keywords: fibrinogen, quality control, protein, Slovenia, CryoSeal, pH, spectrophotometry, Bradford protein assay. Introduction An injury or severe trauma can be considered as a main cause of death in several cases. Since many injuries or traumas lead to bleeding conditions, some of the diseases cannot allow organism to stop bleeding and heal itself. Therefore, there is a need in accurate surveillance and designing solutions to cure people suffering from difficult disorders such as massive hemorrhage, acidosis, hypothermia, coagulopathy, hyperfibrinogenemia, thromboembolism, etc. [1]. According to some data, these disorders might be the result of coagulation complications which are highly associated with an acute phase proteins like fibrinogen [2]. Since fibrinogen is considered as a precursor of coagulation process, unstable bleeding can be the reason of fibrinogen deficiency [2]. Fibrinogen is a glycoprotein which produces in liver and can be found in blood plasma [3]. It is activated by thrombin and blood coagulation factor XIII in the area of trauma or injury forming fibrin clot which in turn stops bleeding [3]. There are several methods to isolate and apply this glycoprotein to patients. However, all known methods can extract fibrinogen only in small concentrations. This article will represent a comparative analysis of quality control of two fibrinogen obtained from human blood plasma by different methods. First method for the extraction of fibrinogen from blood plasma was accomplished by ethanol precipitation. The second method was performed by using an automated device known as CryoSeal FS System that produces fibrin sealant from which fibrinogen was separately extracted. Since isolation of fibrinogen was done in Kazakhstan, the main laboratory work was carried out in the faculty of pharmacy at the University of Ljubljana in 2016. The main purpose of an analysis was to define which method of extraction of fibrinogen from human blood plasma is suitable for remaining protein’s pharmacological activity. Materials and Methods For pH identification : Metrohm/Brinkmann 691 pH Meter, Type 1.691.0020 Electrolyte solution c(KCl) = 3 mol/L, Buffer solution pH = 4.00, Buffer solution pH = 7.00, Buffer solution pH = 9.00 Procedure : Fibrinogen samples were dissolved in distilled water and mixed in laboratory shaker. After electrode in pH meter was calibrated by buffer solutions, the pH of dissolved fibrinogen samples in beakers was calculated by pH meter. For Bradford protein assay (Bradford test) : BSA (bovine serum albumin) standard solution, c = 2 mg/ml purified water