Int. J. of Life Sciences, 2017, Vol. 5 (3): 425-437 ISSN: 2320-7817| eISSN: 2320-964X © 2017 |IJLSCI www.ijlsci.in 425 Comparative study to evaluate in vivo and in vitro antioxidant potential of processed and unprocessed Curcuma logna L. using Allxon induced diabetic rats Parashare VM 1 , Nikam S 1* Nade VS 2 , Pande Shishir 3 1 Department of Biochemistry, KTHM College, Gangapur Road, Nashik (MH), India 2 Department of Pharmacognosy, MVPS’s College of Pharmacy,Nashik (MH), India. 3 Department of Rasshastra & B.K., ASS Ayurved Mahavidyalaya, Nashik (MH), India * Corresponding author e-mail: parashare@gmail.com, Manuscript details: ABSTRACT Received: 14.07.2017 Revised :16.08.2017 Accepted: 16.09.2017 Published : 27.09.2017 Editor: Dr. Arvind Chavhan Cite this article as: Parashare VM, Nikam S Nade VS and Pande Shishir (2017) Comparative study to evaluate in vivo and in vitro antioxidant potential of processed and unprocessed Curcuma logna L. using Allxon induced diabetic rats; International J. of Life Sciences, 5 (3): 425-437. Copyright: © 2017| Author (s), This is an open access article under the terms of the Creative Commons Attribution-Non-Commercial - No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non- commercial and no modifications or adaptations are made. The present study was aimed to investigate in vivo, in vitro antioxidant activity of the processed curcuma Longa L. turmeric (PT) and unprocessed curcuma Longa L. (UPT). Both the extract are subjected to qualitative phytochemical screening. In in-vitro antioxidant properties total Phenolic content in each extract was determined using Gallic acid as a standard, calibration curve was prepared. The result is expressed as mg of Gallic acid equivalent per gram of extract. For unprocessed turmeric (UPT) and processed turmeric (PT) the values were 2.4 mg of GAE/g and 2.1mg of GAE/g. respectively. In in-vitro antioxidant properties total flavonoid content was determined in each extract using Quercetin as reference standards. Total flavonoid content in methanolic extract of UPT and PT in terms of quercetin equivalent (QE) was 152.25mg/100 g and 37.98mg/ 100 g of dry extract respectively. Total antioxidant activity of UPT and PT extract in terms of ascorbic acid equivalent (AAE) was 6.394 and 3.922 mg/ ml of extract respectively. In- vivo study involved five groups of each comprising six Wistar rats (250 - 300 kg) and labelled as Hyperglycemic, Normal, Standard drug Glipizide, UPT treated and PT treated. Besides the Normal group Alloxan was administered in all animals as a single dose (50 mg/kg, i.v.) to induce diabetes. A dose of 300 mg of processed and unprocessed turmeric/kg of body weight was evaluated using acute toxicity test. It is orally administered daily for four weeks after induction of diabetic. The fasting blood glucose level was measured. The animal whose glucose level did not rise to more than 250mg/dl was rejected. Only animals that presented with glycemic levels equal to or above 250 mg/dl were submitted to treatments, which consisted of a daily administration of the UPT and PT turmeric for 21days. It was considered as a first day and blood samples (Approximately 0.5ml) were collected from rats by puncturing the retro orbital sinus, under mild ether anesthesia on 1 st ,7 th , 14 th 21 st day for glucose estimations using GOD-POD method. At the same time urine sugar was estimated using Diastic. At the end of the study all groups rats pancreases were removed, RESEARCH ARTICLE