Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech A promising laccase immobilization approach for Bisphenol A removal from aqueous solutions Fatiha Lassouane a,b, ⁎ , Hamid Aït-Amar a , Saïd Amrani c , Susana Rodriguez-Couto d,e,f, ⁎ a Université des Sciences et de la Technologie Houari Boumediene (USTHB), Laboratoire des Sciences du Génie des Procédés Industriels, Faculté de Génie Mécanique et de Génie des Procédés, BP 32, El-Alia 16111, Algiers, Algeria b Centre de Développement des Energies Renouvelables, CDER, 16340 Algiers, Algeria c Université des Sciences et de la Technologie Houari Boumediene (USTHB), Laboratoire de Biologie des Sols, Faculté des Sciences Biologiques, El-Alia 16111, Algiers, Algeria d Ceit-IK4, Water and Health Division, Paseo Manuel de Lardizábal 15, 20018 Donostia-San Sebastian, Spain e Universidad de Navarra, Tecnun, Paseo Manuel de Lardizábal 13, 20018 Donostia-San Sebastian, Spain f IKERBASQUE, Basque Foundation for Science, María Díaz de Haro 3, 48013 Bilbao, Spain GRAPHICAL ABSTRACT ARTICLE INFO Keywords: Laccase Bisphenol A Crosslinking Alginate beads Trametes pubescens ABSTRACT The immobilization of crude laccase from Trametes pubescens by glutaraldehyde crosslinking prior to entrapment into Ca-alginate beads increased the immobilization yield by 30% and reduced the leaking by 7-fold compared to the immobilization with no crosslinking. The performance of the newly developed biocatalyst to degrade Bisphenol A (BPA) from aqueous solutions was tested. Thus, operating at optimal conditions (i.e. pH 5, 30 °C, 20 mg L -1 BPA and 1500 U L -1 laccase), a BPA removal higher than 99% in 2 h was achieved. This value is higher than those reported to date for BPA removal by immobilized laccases. In addition, the biocatalyst was able to remove BPA in 10 successive batches with an efficiency higher than 70% at the end of the last batch. BPA adsorption on the alginate beads was negligible, therefore BPA removal was only due to laccase action. Moreover, Fourier-transform Infrared (FTIR) spectroscopy suggested BPA transformation by laccase. https://doi.org/10.1016/j.biortech.2018.09.129 Received 1 August 2018; Received in revised form 24 September 2018; Accepted 25 September 2018 ⁎ Corresponding authors at: Université des Sciences et de la Technologie Houari Boumediene (USTHB), Laboratoire des Sciences du Génie des Procédés Industriels, Faculté de Génie Mécanique et de Génie des Procédés, BP 32, El-Alia 16111, Algiers, Algeria (F. Lassouane). Ceit-IK4, Water and Health Division, Paseo Manuel de Lardizábal 15, 20018 Donostia-San Sebastian, Spain (S. Rodriguez-Couto). E-mail addresses: flassouane@usthb.dz (F. Lassouane), srodriguez@ceit.es (S. Rodriguez-Couto). Bioresource Technology 271 (2019) 360–367 Available online 27 September 2018 0960-8524/ © 2018 Elsevier Ltd. All rights reserved. T