Madridge J Anal Sci Instrum. ISSN: 2638-1532 56 Volume 3 • Issue 1 • 1000111 Madridge Journal of Analytical Sciences and Instrumentation Research Article Open Access Manipulation of Different ratio Dependent approaches for Determination of Pravastatin in the presence of Pioglitazone in Pharmaceutical Preparation Nasr M. El-Abasawi, Khalid A.M. Attia, Ahmad A. M, Abo-serie and Ashraf Abdel-Fattah* Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt Article Info *Corresponding author: Ashraf Abdel-Fattah Pharmaceutical Analytical Chemistry Department Faculty of Pharmacy Al-Azhar University 11751 Nasr City, Cairo Egypt Tel: +201009408060 Email: ashraf.abdelfattah@hotmail.com Received: February 16, 2018 Accepted: March 3, 2018 Published: March 9, 2018 Citation: El-Abasawi NM, Attia KAM, Aboserie AAM, Morshedy S, Abdel-Fattah A. Manipulation of Different ratio Dependent approaches for Determination of Pravastatin in the presence of Pioglitazone in Pharmaceutical Preparation. Madridge J Anal Sci Instrum. 2018; 3(1): 56-61. doi: 10.18689/mjai-1000111 Copyright: © 2018 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Published by Madridge Publishers Abstract Objective: This study aimed to develop three simple UV spectrophotometric methods for determination of pravastatin sodium in the presence of pioglitazone hydrochloride without previous separation. Methods: Manipulating ratio spectra were developed for the interested methods. The first method is the ratio subtraction using 16 µg/mL of pioglitazone hydrochloride as a divisor. The second method is an amplitude modulation using the normalized spectrum of pioglitazone hydrochloride as a divisor (1 µg/mL) the peak amplitudes of ratio spectra were measured at isoabsorptivity point 251.4 nm. The third method is the ratio difference using 14 µg/mL of pioglitazone hydrochloride as a divisor, where the peak amplitudes of ratio spectra were measured at 240 and 246.4 nm. The results and discussion: The calibration curve is a linear over the concentration range of 2-20 µg/mL, the proposed methods were validated according to International Conference on Harmonization (ICH) guidelines and successfully applied for the determination of pravastatin sodium in the presence of pioglitazone hydrochloride in their combined pharmaceutical formulation, Pravazon® capsules with high sensitivity. Conclusion: The proposed three methods are simple, rapid, economical, accurate and precise for determination of pravastatin sodium in the presence of pioglitazone hydrochloride without previous separation. Keywords: Pravastatin Sodium; Pioglitazone Hydrochloride; Ratio Subtraction; Amplitude Modulation; Ratio Difference. Introduction Pravastatin sodium (PRV), (Figure 1a), is sodium (3R,5R)-7-{(1S,2S,6S,8S,8aR)- 1,2,6,7,8,8a-hexahydro-6-hydroxy-2-methyl-8-[(S)-2-methylbutyryloxy]-1-naphthyl}- 3,5-dihydroxyheptanoate [1,2]. It is a selective and competitive inhibitor of 3-hydroxy-3- methylglutaryl -coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme that converts HMG-CoA to mevalonate, a precursor of cholesterol [3]. PRV is a member of the class of statins, used to treat hypercholesterolemia and related conditions and to prevent cardiovascular disease. It increases the number of hepatic low density lipoprotein (LDL) receptors on the cell surface to enhance uptake and catabolism of LDL. Secondly, PRV inhibits hepatic synthesis of very low density lipoprotein (VLDL), which reduces the total number of VLDL and LDL particles [4]. Pioglitazone hydrochloride (PGZ), (Figure. 1b), is 5-[[4-[2-(5-Ethyl-2-pyridinyl) ethoxy]phenyl]methyl]-2,4-thiazolidinedione hydrochloride [5]. It is a thiazolidine-dione oral antidiabetic agent that improves insulin sensitivity. It is used in the treatment of ISSN: 2638-1532