International Journal of
Life Sciences
International Peer Reviewed Open Access Refereed Journal
Special Issue A 9: January 2018
UGC Approved Journal No 48951
Original Article Open Access
National Conference on “Emerging Trends in Mycotechnology” (NCETM-2018) | 79
Isolation and Characterization of dandruff causing fungi &
effect of some plant extracts on it
Nirmalkar Vaishali*
1
and Randhir Nayan
2
1
K.M.E Society’s G. M. Momin Women’s College, Bhiwandi, Maharashtra
2
M.V.P Samaj’s KTHM College, Nashik, Maharashtra
*corresponding author, email: vaishu_p2025@yahoo.co.in
Manuscript details: ABSTRACT
Available online on
http://www.ijlsci.in
ISSN: 2320-964X (Online)
ISSN: 2320-7817 (Print)
Editor: Dr. Arvind Chavhan
Cite this article as:
Nirmalkar Vaishali and Randhir
Nayan (2018) Isolation and
Characterization of dandruff
causing fungi & effect of some
plant extracts on it, Int. J. of. Life
Sciences, Special Issue, A9: 79-83.
Copyright: © Author, This is an
open access article under the terms
of the Creative Commons
Attribution-Non-Commercial - No
Derives License, which permits
use and distribution in any
medium, provided the original
work is properly cited, the use is
non-commercial and no
modifications or adaptations are
made.
Yeast called Malassezia is a causative agent of Seborrheic dermatitis or
dandruff. It was isolated from the scalp of human individuals and
investigated further. Suitable biochemical tests were performed for the
characterization of the organism focusing on its ability to produce
Catalase, Urease, utilization of tweens, liquefaction of gelatin and
assimilation of different carbohydrates. The organism after its isolation
and characterization was further investigated for its susceptibility
towards 20 different plant extracts.
Keywords: Seborrheic dermatitis, Malasezzia, mDixons agar, antifungal
activity, medicinal plants.
INTRODUCTION
Fungi are ancient troublemakers, but the suspicion that they might
cause disease is about 100 years old. Seborrheic dermatitis /dandruff is
a condition where flaky skin of scalp sheds off. Louis Charles Malassez
first saw yeast like substance lurking in the scales of a patient with
Seborrheic dermatitis. Malassezia furfur (Pitryosporum ovale), is the
fungus which plays an important role in Seborrhoeic dermatitis
(Faergemann et al., 1996). The genus Malassezia comprises lipophilic
yeasts found in the normal flora of human skin and other mammals.
These yeasts were described as being associated with Pityriasis
Versicolor (PV) lesions. The taxonomy and nomenclature of the genus
Malassezia was controversial for many decades. Nine of the 13 species
within the genus, M. furfur, M. sympodialis, M. globosa, M. restricta, M.
slooffiae, M. obtusa, M. dermatis, M. japonica, and M. yamatoensis, are
associated with normal human flora and pathologies. Four species, M.
pachydermatis, M. nana, M. equina, and M. caprae, are associated with