www.tjprc.org editor@tjprc.org THE EXPERIMENTAL WOUND HEALING USING COFFEE POWDER AND HONEY COMPARED TO NPWT TAMMY HERLIANI, FAUZAN AKBAR YUSYAHADI, HENDRO SUDJONO YUWONO, ISMET M. NOOR, KINCE SAKINAH & TONY DJAJAKUSUMAH School of Medicine, Universitas Islam Bandung (UNISBA), Bandung, Indonesia ABSTRACT Today the Negative Pressure Therapy (NPWT) applied to treat wounds. The procedure needs to criticize because not simple, expensive, use only by doctors, and limited availability. The wound treatment using Robusta coffee powder compared to honey, NPWT, and control groups. The wound sized 2cmx2cmx0.20 cm made at the back skin. Each wound infected by 1 ml. of a bacterial suspension of Staphylococcus aureus ATCC 25923. The grade of epithelialization, angiogenesis, collagenization, on the 7 th day, 14 th day, were observed. The randomized observational cohort analytic research conducted in 250 gram-Wistar rats divided into three groups, each group of 5 rats. On the 7 th day and the14 th -day, the wound biopsied and put into formalin solution. The epithelial cell growth inspected using 200 x-magnification. The healing parameter using the grade of epithelialization 3=normal, grade 2=low epithelization, 1=no growth; grade of angiogenesis 3=more than two new blood vessels; 2=one to two blood vessels; 1=no new blood vessel; grade of collagenization 3=more than normal collagen density; 2=the same with normal collagen density; 1=low than normal collagen density. Data analysis using the Kruskal-Wallis test. Statistics analyzed using SPSS 24.0; p-value < 0.05 considered significant. The grade of epithelialization, angiogenesis, collagenization were not statistically significant in the 7 th day groups of control, Robusta coffee, honey, NPWT, with p=1.000. In the 14 th day groups of control, Robusta coffee, honey, NPWT, the epithelialization, and angiogenesis have p=1.000, and the collagenization on the 14 th day has p=0.271. CONCLUSIONS The wound healing using coffee powder or honey was not different compared with NPWT. KEYWORDS: Wound Healing, Staphylococcus Aureus ATCC 25923, Robusta Coffee, Honey & NPWT Received: Sep 13, 2018; Accepted: Oct 03, 2018; Published: Oct 29, 2018; Paper Id.: IJMPSDEC20183 INTRODUCTION The wound care method in this modern time is among others using Negative Pressure Wound Therapy (NPWT) which developed in the world since the 1980s. 1 The NPWT also called subatmospheric pressure therapy, vacuum sealing, vacuum pack therapy, and sealing aspirative therapy, is applied to acute and chronic wounds. 1 The wound care method needs to evaluate because the NPWT procedure is complicated, high in price, use only by doctors, and limited availability. 1,2 Negative pressure has proven its ability to suck infected wound fluid, stimulates the formation of granulation tissue to accelerate healing. 1,2 Coffee and honey powder have long been known as traditional wound treatments that are effective, inexpensive, and are procedures that are easily done by anyone Original Article International Journal of Medicine and Pharmaceutical Science (IJMPS) ISSN (P): 2250-0049; ISSN (E): 2321-0095 Vol. 8, Issue 6, Dec 2018, 19-22 © TJPRC Pvt. Ltd.