Available online at www.derpharmachemica.com ISSN 0975-413X CODEN (USA): PCHHAX Der PharmaChemica, 2016, 8(19):657-665 (http://derpharmachemica.com/archive.html) 657 In Vitro antifungal potential of Acinetobacter baumannii and determination of its chemical composition by gas chromatography-mass spectrometry Mohanad Jawad Kadhim Department of Genetic Engineering, Al-Qasim Green University, Iraq _____________________________________________________________________________________________ ABSTRACT The aims of this research were analysis of the bioactive chemical products and evaluation of antibacterial and antifungal activity. Fifty one bioactive compounds were identified in the methanolic extract of Acinetobacter baumannii. GC-MS analysis of Acinetobacter baumannii revealed the existence of the Malonic acid ,mononitrile , monothioamide, Oxime-,methoxy, 1,2,3-Propatriol, 1-indol-4-yl ; Propenoic acid , 3-[(phenylmethyl)sulfonyl]- , methyl ester, Cis-Pinen-3-ol, 3-Benzoylmethyl-3-hydroxy-5-nitro-2-indolinone, 3-(1-Cyclopentenyl)furan , 3- Benzoylmethyl-3-hydroxy-5-nitro-2-indolinone, 2-Phenylethanamidine, Cyclohexane, 1,3-butadienylidene- , Benzenemethanol, 2-(2-aminopropoxy)-3-methyl, 4H-1-Benzopyran-4-one, 6,7-dimethoxy-3-phenyl-, Cyclopentolate, Levmetamfetamine, Ethanamine,2-(2,6-dimethylphenoxy)-N-methyl, (3-Ethoxy-4,5-dihydro- isoxazol-5-ylmethyl)-amine, DL-Leucine , N-glycyl- , 3,6,9,12-Tetraoxatetradecan-1-ol , 14-[4-(1,1,3-tetramethyl , Phenethylamine , N-hexyl , Dithiocarbamate ,S-methyl-,N-(2-methyl-3-oxobuty , N-Methyl-4-phenyl-butyramide , Hydrazinecarboxamide ,2-[3-cyclohex-3-en- , Hydrazinecarboxamide,2-[3-cyclohex-3-en-, Pterin-6-carboxylic acid , Benzofuran-2-one , 4-amino-2,3-dihydro-, Cyclopentanecarboxylic acid , 1-phenyl -,2-(diethylamino)ethy, Ethanamine , 2-(2,6-dimethylphenoxy)-N-methyl-, Diaziridinone, (1,1-dimethylethyl)(1,1-dimethyl-2-phenylethyl)-, dl-Alanyl-dl-serine, 4-Ethoxy-6-methylhexahydropyrimidin-2-thione, 4H,5H-Pyrano[4,3-d]-1,3-dioxin , tetrahydro- 8a-methyl-, Eicosanoic acid, phenylmethyl ester, Pyrrolo[1,2-a]pyrazine-1,4-dione , hexahydro-, Dibutyl phthalate, Pyrrolo[1,2-a]pyrazine-1,4-dione , hexahydro-3-(2-methylpropy, Pterin-6-carboxylic acid, Cytidine , 5-methyl-, 12- Methyl-oxa-cyclododecan-2-one, 3,7-Diazabicyclo[3,3,1]nonane, 9,9-dimethyl-, Actinomycin C2, 4-Azepan-1-yl- oxazolidin-2-one, 4-Carbamoyl-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl, i-Propyl 9-tetradecenoate, 4,4,6- Trimethyl-2-(N-methyl-m-chlorophenylamino)-5,6-dihydro, 2-Butenoic acid , 4-(morpholin-4-yl)-, methyl ester, 2,5- Piperazinedione , 3,6-bis(2-methylpropyl)-, l-f Leucyl -d-leucine, 3H-3a-Azacyclopenta[a]undene -2-carbonitrile ,3- oxo-1-(piper, and Phthalic acid , di(2-propylpentyl) ester. The results of anti-fungal activity produced by Acinetobacter baumannii showed that the volatile compounds were highly effective to suppress the growth of Aspergillus flavus. Acinetobacter baumannii produce many important secondary metabolites with high biological activities. Keywords: Acinetobacter baumannii, Antifungal activity, gas chromatography-mass spectrometry, Metabolites. _____________________________________________________________________________________________ INTRODUCTION Acinetobacter baumannii is an opportunistic, Gram-negative, cocco-bacillus which is known to cause nosocomial infections like septicaemia, bacteraemia, pneumonia, wound sepsis, endocarditis, meningitis, and urinary tract infections [1, 2]. Acinetobacter are usually found in the hospital environment and infect patients who are hospitalized from a long period of time with severe underlying diseases, are immunosuppressed, or subjected to invasive procedures and treated with broad-spectrum antibiotics [3]. During outbreaks along with one or more epidemic Acinetobacter clones, there exists anendemic strain which makes it difficult to identify and control their transmission [4]. Reports on resistance mechanisms in A. baumannii mainly focused on outer membrane impermeability, production of beta lactamases and production of efflux pump. However, equally important for