Sivakumar et al Journal of Drug Delivery & Therapeutics. 2018; 8(6):195-200 ISSN: 2250-1177 [195] CODEN (USA): JDDTAO Available online on 15.11.2018 at http://jddtonline.info Journal of Drug Delivery and Therapeutics Open Access to Pharmaceutical and Medical Research © 2011-18, publisher and licensee JDDT, This is an Open Access article which permits unrestricted non-commercial use, provided the original work is properly cited Open Access Research Article In-vitro cytotoxicity of java tea mediated selenium nanoballs against L6 cell lines Chitra Sivakumar 1* , Karthikeyan Jeganathan 2 1 Research Scholar, Research and Development Centre, Bharathiar University, Coimbatore, India 2 Associate Professor and Head, Department of Biochemistry, (PG and Research) Kongunadu Arts and Science College, (Autonomous), Coimbatore, India ABSTRACT The emergence of Nanotechnology has provided a catholic research in recent years by intersecting with assorted branches of science and forming collision on all forms of life. At present there has been a prodigious excitement in the field of Nano pharmacology to seek the role of Nano selenium in individual healthcare and explains how selenium is a double edged sword in the pathologies of chronic diseases like Diabetes mellitus because of their inferior toxicity and ability to gradually release the bioactive principle and free selenium after ingestion. Thus the present study is an embryonic attempt and was aimed at the adroitful synthesis of selenium nanoballs from the aqueous extract of well known herbal tea leaves namely Java tea ( Orthosiphon stamineus) using selenious acid solution. The synthesized Selenium nanoparticles were then subjected to various characterization techniques such as UV, FTIR, FESEM, EDAX and Zeta potential respectively. Finally the green synthesized SeNps were tested for their cytotoxic effect against L6 rat skeletal muscle cell lines. The pre clinical studies are underway to prove the insulin mimic activity of the selenium nanoparticles. Keywords: Selenium Nanoparticles, Java tea, L6 cell lines. Article Info: Received 03 Oct, 2018; Review Completed 06 Nov 2018; Accepted 09 Nov 2018; Available online 15 Nov 2018 Cite this article as: Sivakumar C, Jeganathan K,In-vitro cytotoxicity of java tea mediated selenium nanoballs against L6 cell lines, Journal of Drug Delivery and Therapeutics. 2018; 8(6):195-200 DOI: http://dx.doi.org/10.22270/jddt.v8i6.2046 *Address for Correspondence: Chitra Sivakumar, Research Scholar, Research and Development Centre, Bharathiar University, Coimbatore, India 1. INTRODUCTION The field of Nanotechnology devours great enthusiasm in recent years because of its anticipated impact on science, industry, economy and our everyday life. Today nanoparticles of both metallic and non metallic origin are under research and development in various fields of biology and therapeutics 1. Due to the inimitable characteristics exhibited by nanoparticles, they are employed in nanomedicine and nanotherapeutics which are the supreme aspects of nanotechnology to be implemented in human health. Metal nanoparticles are vital part of future nanotherapeutics which has wide-ranging applications in diverse areas such as chemistry, physics, and biomedical and material sciences 2. They have tremendous applications in the area of catalysis, optoelectronics, diagnostic biological probes and display devices 3 . Production of nanoparticles can be achieved through conventional chemical methods and physical methods 4,5. While chemical approaches are the most popular methods for the production of nanoparticles some chemical methods cannot avoid the use of toxic chemicals in the synthesis protocol. Engaging plants in the synthesis of nanoparticles has drawn more interest of workers because it provides single step biosynthesis process, can be advantageous over other biological processes by eliminating the elaborate process of maintaining cell culture 6 . Plants also tender a superior option for synthesis of nanoparticle, as the protocols involving plant sources are free from toxicants; furthermore, natural capping agents are readily supplied by the plants. Selenium (Se), belonging to group 16 of the periodic table is well known for its photoelectric and semiconductor properties. These nanoparticles show biological activity and good adsorptive ability due to interaction between the nanoparticles and NH, C O, COO− and C N groups of proteins 7 . Selenium nanoparticles have also been developed for applications in medical diagnostics 8 . Thus selenium nanoparticles caused the great interest of researchers and a variety of synthesis methods have been exploited 9 . Biogenic synthesis of Se nanoparticles is frequently achieved by reduction of selenate/selenite in presence of bacterial proteins or plant extracts containing phenols,