ISSN 2348-313X (Print) International Journal of Life Sciences Research ISSN 2348-3148 (online) Vol. 7, Issue 1, pp: (240-248), Month: January - March 2019, Available at: www.researchpublish.com Page | 240 Research Publish Journals EVALUATION OF EGG HATCHABILITY AND LARVAL MORTALITY OF METHANOLIC EXTRACTS OF CHROMOLAENA ODORATA AND ANNONA SQUAMOSA ON MELOIDOGYNE INCOGNITA 1 Kamatchi K., 2 Nattuthurai N., 3* Krishnamoorthy S. 1 PG and Research Department of Zoology, Vivekananda College, Madurai, Tamil Nadu, India Abstract: Chromolaena Odorata and other associated species of Annona Squamosa have been reported be nematicidal. This research was intended at providing more information on the nematicidal activity of Chromolaena odorata and exposure the nematicidal action of Annona squamosa. Ten grams each of the powders were drenched in cold 100 ml of methanol for one week to produce 100,000 mg/kg stock solution, filtered and concentrated. One ml of nematode suspension that contained 60 eggs of M. incognita extracted with sodium hypochlorite, one ml of nematode suspension that contained 60 one week old second-stage juveniles dispensed into glass blocks that contained one ml of the extracts. Percentage inhibition and juvenile mortality were estimated. Dry powders were analyzed for Infrared and phytochemicals. Water extracts inhibited egg-hatch of Meloidogyne incognita (25-100%) and killed the juveniles (2.3-100%). Water extracts, 20,000 mg/kg, 30,000 mg/kg as from fourth day had 100% mortality of the juveniles. The IR revealed alcohols, amines, unsaturated/aromatic compounds and phenols. The phytochemicals identified were saponins, tannins, phenols, alkaloids, flavonoids, anthraquinones and cardenolides. Saponins were of the highest amounts followed by tannins and phenols. These botanicals were inhibitors of egg- hatch, lethal to juveniles of Meloidogyne incognita and might be attributed to the phytochemicals. Keywords: Meloidogyne incognita, Egg hatchability, Larval mortality, Nematicidal activity. 1. INTRODUCTION Plant-parasitic nematodes are found wherever plants grow and are known to be among the limitations of producing food for man and his livestock. The predictable annual yield losses due plant-parasitic nematodes in the world’s major crops are documented about 12.3% and 14% in the developing countries (Ngangbam and Devi, 2012). The root-knot nematodes Meloidogyne spp. such as Meloidogyne incognita is noteworthy pest of crops ranging from vegetables, cereals, legumes to perennial crops (Sikora and Fernandez, 2005). The root-knot nematodes, due to their high reproductive potential and wide host ranges are disreputably difficult to handle. Meloidogyne spp. requires 99.9% manage in order to put off the succeeding build up of damaging populations (Chaudhary and Kaul, 2013). The root-knot nematode lays eggs in the protective gelatinous matrix (Abad et al., 2009). The infective stage of the root-knot nematode is the second-stage juvenile (J2) which hatches out from the egg, which invade the roots by active incursion (Curtis, 2008). The