Bas.J.Vet.Res.Vol.15,No.2,2016 . ISI Impact Factor:3.461 360 ISOLATON AND IDENTIFICATION SOME BACTERIAL CAUSES OF LUNG ABSCESSESSHEEP BY CHROMOGENIC MEDIA Nabeel Ahmed Al -Anbagi College of Veterinary medicine ,University of kufa,kufa ,Iraq. (Received 25 February 2016,Accepted 10 May 2016) Key word : Abscesses ,Chromagar, sheep. ABSTRACT The study carried to isolation and identification some bacterial causes of lung abscesses in the sheep in Najaf province by Orientation chromagar. Forty two lung abscess were collected fromdied (10) and slaughtered sheep(32). The result of bacterial isolation appeared 32 bacterial isolates included 18 (56.2%) Gram positive bacteria and 14(43.7%) Gram negative bacteria according morphology and color of colony and confirmed by classical biochemical test .The Staphylococcus aureus were predominant bacteria 15(46.8%) , Escherichia coli 9 (28.1 %), Klebsiella pneumonia 5 (15.6 %) and Staphylococcus saprophyticus 3 (9.3%). INTRODUCTION lung the major organs of respiratory system, is susceptiblefor several infectious and non infectious agents cause pathological change in farm animals, among these agents, pneumonia(acute and chronic ) causes weakness and fate animal leading to high economic loss (1,2). The respiratory system diseases such as pneumonia and unspecific toxemias were the most common causes of deaths in sheep(3). The respiratory diseases in sheep complex causes such bacteria virus ,the bacterial causes of pneumonia is most frequent and lead to high mortality and economic loss( 3). Abscess is a necrotizing lesion characterized by a pus-filled cavity(4). The most common causes of lung abscesses due to aspiration are some bacteria such as Staphylococcus aureus and Klebsiella Mycobacterium tuberculosis. (5). Orientation chromagar is one of a number of new chromogenic agars , it used for identification of some Gram negative bacteria and some Gram positive bacteria (6) the basis of