International Journal of Advanced Engineering Research and Science (IJAERS) [Vol-6, Issue-6, June- 2019] https://dx.doi.org/10.22161/ijaers.6.6.85 ISSN: 2349-6495(P) | 2456-1908(O) www.ijaers.com Page | 731 Formulation of an Antioxidant Cosmetic Cream Containing Coffea arabica Fractions Monik Ellen dos Santos Diamantino 1 , Anny Carolinny Tigre Almeida Chaves 2 , Daniel de Melo Silva 1 , Gisele da Silveira Lemos 1 , Raphael Ferreira Queiroz 3,* 1 Department of Chemical and Exact Sciences; State University of Bahia Southwest, Rua José Moreira Sobrinho, S/N, Jequiezinho, CEP 451206190, Jequié, Bahia, Brazil. ²Biotechnology Graduate Program; State University of Santana Fair, Av. Trans Nordestina, S/N, Novo Horizonte, CEP 44036900, Feira de Santana, Bahia, Brazil. 3 Department of Natural Sciences, State University of Bahia Southwest, Estrada do Bem Querer, Km 04, S/N, Universitário, CEP 45031900, Vitória da Conquista, Bahia, Brazil. *Corresponding author: Abstract —Coffee is a very popular drink, which is traditionally used to supplement meals and for hedonistic purposes beyond of its functional and health properties. The coffee provides a multitude of bioactive by-products that can be used for the formulation of dermocosmetics. In this context, this work aimed to develop an emulsion using extracts of Coffea arabica as well as evaluate its physicochemical stability and antioxidant activity. Two formulations were developed with incorporation of different fractions of green seeds from Coffea arabica. Then, both formulations were submitted to extremes temperatures (5 and 45 o C) for 15 days, and pH, electrical conductivity, organoleptic characteristics and presence of viable microorganisms were determined. Total phenolic content was estimated by Folin-Ciocalteu method. Afterwards, fractions and cream were tested by 2,2- diphenyl-1-picrylhydrazyl (  DPPH) radical scavenging assay. The formulation 2 showed no instability for all parameters evaluated. The hexane, glycolic, methanol, chloroform and ethyl acetate fractions, and cream at 150 μg/mL deactivated  DPPH radical at 24.7, 51.8, 86.2, 89.5, 90.9 and 79.8%, respectively. Furthermore, the emulsion kept its antioxidant activity even under accelerate tests. The results confirmed the stability of cream containing coffee fractions, suggesting its use as natural antioxidant cosmetic cream and further experiments to evaluate its photoprotective potential. Keywords— coffee; cosmetics; antioxidant; cosmetic cream; stability test. I. INTRODUCTION According to International Coffee Organization data (ICO, 2016), in the period from 1996 to 2015, Brazil was the world’s largest coffee producer (varying from 20.8% to 39.4% of the world production) and the third world consumer (after the European Union and the USA), but second if considering the individual countries of the European Union. In Brazil, between 2016/2017, there was a total production of 49,640 thousand bags of 60 kg and a total domestic consumption of 20,500 thousand bags of 60 kg of coffee. The State of Bahia has played an important role in the production of Arabica coffee and is the fourth largest producer in Brazil, contributing to Brazilian position as largest producer and exporter in the last three decades. Compared to other producing states, Bahia has been standing out by employing high technology and obtaining excellent productivity rates (Bliska et al ., 2009; Frederico, 2012). Besides to be a drink, the search for alternatives to coffee has established several by-products such as coffee oils (Tango, 1971), which attracts attention from cosmetic industry as a source of bioactive products (Esquivel, Jiménez, 2012). Since they are rich in phytosterols and essential fatty acids, coffee oils are rapidly absorbed and have good adhesion in cutaneous applications, whose properties go beyond dermal emollients (Wagemaker et al ., 2012). The major unsaturated fatty acids present in coffee oils are linoleic acid and palmitic acid (Wagemaker et al ., 2011). They are often employed in cosmetic products to improve skin appearance, and especially treat aging signs (Webber, Ribeiro, Velásquez, 2005; Nosari et al., 2015). In fact, vegetable oils have been widely used as a basic