~ 95 ~ The Pharma Innovation Journal 2018; 7(11): 95-102 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2018; 7(11): 95-102 © 2018 TPI www.thepharmajournal.com Received: 19-09-2018 Accepted: 20-10-2018 J Shyam Sundar VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India R Gloria Jemmi Christobel (1) VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India (2) V. V. Vanniaperumal College for Women, Virudhunagar, Tamil Nadu, India N Kasi Selvi V.V. Vanniaperumal College for Women, Virudhunagar, Tamil Nadu, India MP Abirami VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India Dr. Shila Samuel VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India Correspondence R Gloria Jemmi Christobel Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India Efficient green synthesis of silver nanoparticles from Caesalpinia bonducella seeds and its antibacterial and cytotoxic effects: An in vitro study J Shyam Sundar, R Gloria Jemmi Christobel, N Kasi Selvi, MP Abirami and Dr. Shila Samuel Abstract The present study reports the successful synthesis of silver nanoparticles (Ag NPs) by plant-mediated green route and antimicrobial and anticancer activity investigated. The seed extract of locally sourced Caesalpinia bonducella acted as the reducing agents/capping agents. The CB seed AgNPs characteristics elucidated under UV–visible spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy. The antimicrobial activity of the synthesized nanoparticles was tested against various pathogenic bacteria. Anticancer effect in Human breast cancer cell line MCF-7 was investigated. The synthesized AgNPs exhibited a maximum absorption at 455 nm and the Fourier transform infrared spectroscopy analysis confirmed the conversion of Ag+ ions to AgNPs due to the reduction by capping materials such as flavonoids and alkaloids of seed extract. The HR-TEM analysis revealed that they are spherical ranging 40nm. Significant growth inhibitions were found using analysis of variance (ANOVA), SPSS statistical tool at P<0.05. The highest activity of CB seed Ag NPs was against P. aeruginosa. Cytotoxicity of biosynthesized AgNPs against in vitro Human breast cancer cell line MCF-7 showed a dose-response activity with an IC50 value of 75μg/ml. Hence, the findings of this research suggest potential applications of the bionanoparticles as a candidate for therapeutic drugs because of their biogenic nature. Keywords: Caesalpinia bonducella seed, CB seed AgNPs, FTIR, TEM, Anti microbial activity, anti cancer activity Introduction The emerging field of nanobiotechnology is envisioned to be one of the most fruitful areas of research in current science. The construction and characterization of noble metal nanoparticles such as gold silver and platinum is an upcoming field of research due to their vital applications in the fields of biotechnology, bioengineering, textile engineering, water treatment, metal- based consumer products and other areas [37] . Due to their utilization in various sectors, biomolecules found in the plant extracts can be employed to reduce metal ions to synthesize nanoparticles (NP) in green synthesis process [33] . Among them one of the most studied nanoparticles is silver nanoparticles, and it has greatly shown anti-neoplastic, anti- inflammatory and anti-bacterial effects in previous studies [15, 16] . Production of silver NPs using different medicinal plants for pharmaceutical and biological applications have been reported [26, 29] . Nowadays treating bacterial infection is risingly complicated because of the ability of the pathogens to develop resistance to available antimicrobial agents and existing antibiotics. Resistant pathogens tend to become broader infection control problems within hospitals and communities as well [42] . Resistant bacteria like Staphylococci, Enterococci, Klebsiella Pneumonia and Pseudomonas sp. are becoming more and more common [51] . To overcome this, novel strategies are required. The favorable approach was the use of natural antimicrobials individually, combination or synergistically and this set forth the way for the use of metal nanoparticles recently. Many studies evidenced that the cytotoxicity of synthesized Ag NPs is allied with their ability to produce reactive oxygen species (ROS) and initiating mitochondrial membrane disruption [3] . This cytotoxic property therefore manifest antitumor effects against lung cancer H1299 cells, breast cancer MCF-7 cells and glioblastoma U-87 cells [11, 10, 30] . AgNPs could be internalized by the cells through Trojan effect and inhibit the RNA polymerase activity and the gene transcription via a direct