International Journal of Enhanced Research in Science Technology & Engineering, ISSN: 2319-7463 Vol. 3 Issue 1, January-2014, pp: (313-318), Impact Factor: 1.252, Available online at: www.erpublications.com Page | 313 Evaluation of Antimicrobial Effect for Chlorhexidine incorporated Gutta Perchausing FTIR Spectroscopy Amer A. Taqa 1 , Rajaa T. Suliman 2 , Enas Y. Shehab 3 13 Department of Dental Basic Science, College of Dentistry, Mosul University, Iraq 2 Department of Conservative Dentistry, College of Dentistry, Mosul University, Iraq ABSTRACT Aims: To evaluate the antimicrobial effects for chlorhexidine incorporated gutta-percha after using FTIR Spectroscopy. Materials and Methods: In this study 80 conventional Gutta-percha (GP) cones the same size (80) were used, first 10cones disinfected by immersion cones with 2%chlorhexidine solution (CHX) for10minutes after that storage in sterile distilled water solution for 24hrs. The chemical incorporation of CHX and GP cones evaluated by using FTIR Spectroscopy. The antimicrobial effect of CHX incorporated gutta percha cones evaluated using the antimicrobial sensitivity test. The results: There is a Chemical incorporation for a 2% Chlorhexidine solution with GP cones were evaluated using FTIR Spectroscopy, antimicrobial effect of CHX incorporated GP (medicated Gutta- percha) indicated that there are inhibition of growth for all tested microorganisms specially for Streptococcus Mutans after 10 minutes immersion of GP cones in 2% CHX solution. Conclusion: Chlorhexidine incorporated gutta- percha offer additional antimicrobial advantages over conventional gutta-percha increasing the wettability and not the effect on mechanical properties of GP cones. Key words: Gutta- Percha, Chlorhexidine, FTIR Spectroscopy. INTRODUCTION The microorganisms are the major etiological agents in pulpal and periapical disease. Two of the main goals of endodontic therapy are the elimination micro-organisms and their products from the root canal system and the prevention of subsequent reinfection. Persisting bacteria or reinfection bacteria may induce or sustain apical periodontitis (1,2) . Guttapercha has been used to fill root canals for a century of years and remain the material of choice in the obturation root canal system. The GPcones taken directly from the manufacture sealed package harbored. Microorganisms were quite low at the time of the opening of the package; clinical use of the packages increased the number of microorganisms contaminating GP cones (3) . Evidence for the antimicrobial activity for guttaperchacones is important, Zinc oxide, which is the major component of gutta percha and responsible for some of the antibacterial properties of cone (4) . The core filling materials should be free from microorganismsin order to avoid contamination. Therefore the endodontic filling materials should have an antimicrobial effect to avoid microorganism growth (2) . Current rootcanal obturation technique involves the use of several chemicals inhibiting the microbial growth and new form of gutta percha cones has an antimicrobial effect without effect on the final outcome of arbitration (5) . Brenda et al. concluded from their study, chemically disinfected substance like chlor hexidine (CHX) has ability to kill vegetative forms of bacteria within short periods of times (6) . Chlorhexidine don’t change gutta percha cone properties after exposure for up to30 minutes when used as disinfectant for obturation materials (GP and reselling cones) (6) . Therefore, this substance (CHX) is less harmful to the structure of guttapercha and 2%CHX don’t produce any changes on gutta percha and resilon surface (6,7,8) .